摘要
[目的]建立藿香正气水中厚朴酚与和厚朴酚的一测多评方法。[方法]以厚朴酚为内标,建立其与和厚朴酚的相对校正因子,用高效液相色谱法进行含量测定,计算厚朴酚、和厚朴酚的含量,对一测多评法的计算值与外标法实测值进行比较。[结果]建立的相对校正因子重现性良好,采用相对校正因子计算的含量与外标法实测值之间无显著性差异。[结论]一测多评法可用于藿香正气水中厚朴酚与和厚朴酚的含量测定,且方法可靠,结果准确,能有效提高检测的效率及节约对照品。
Objective:To establish QAMS for determination of magnolol and honokiol in Huoxiang Zhengqi tincture. [Methods]The relative correction factors of honokiol were established with magnolol as internal indicator, and the content was determined by high performance liquid chromatography(HPLC). Then the RCF was used to calculate the content of the Honokiol. In this way,the calculated value was compared with those measured with the external standard method. [Results]The established relative correction factor had good reproducibility. There was no significant difference between the calculated value by the relative correction factor and the measured value of the external standard method.[Conclusions]QAMS can be used to determine the contents of magnolol and honokiol in Huoxiang Zhengqi tincture. The described method is reliable, accurate, and can effectively improve detection efficiency and save reference substance.
作者
夏丽珍
张敏芳
倪璟雯
Xia Lizhen Zhang Minfang Ni Jingwen(Fujian Sanming Integration of Chinese and Western medicine hospital, Fujian Province, 365001)
出处
《广西中医药大学学报》
2017年第1期49-52,共4页
Journal of Guangxi University Of Chinese Medicine