新疆主要小麦品种(系)中抗条锈病基因的分子检测

Molecular Detection of Stripe Rust-Resistant Genes in Main Wheat Varieties(Lines) from Xinjiang

【目的】选用抗条锈病基因Yr10和1BL/1RS易位的分子标记,明确抗条锈病基因Yr10和1BL/1RS易位在新疆小麦品种(系)中的分布,为新疆小麦抗条锈病品种的合理布局及抗性材料的选育提供理论依据。【方法】利用Yr10及1BL/1RS易位的分子标记,检测其在新疆麦区46份小麦材料中的分布。【结果】在46份供试材料中,新冬17号检测到Yr10基因的存在;新冬24号、新冬46号、MJ307、HMJ555、1112、新紫1号、983-S、新麦211和新麦23检测到1BL/1RS易位,占全部材料的19.57%。【结论】对当前小麦条锈病流行小种条中32号具有较好抗性的Yr10基因,在新疆麦区小麦主栽品种(系)中分布率很低,可能含有Yr10基因的新冬17号在选育抗病材料中具有一定价值,育种中应加强基因Yr10的利用;在选育新品种时需减少1BL/1RS易位的分布频率。

【Objective】The major purpose of the study is to make sure the distribution of stripe rust-resistant genes Yr10 and 1BL/1RS translocation in wheat cultivars or breeding lines in Xinjiang. 【Method】Forty six wheat cultivars and breeding lines collected from Xinjiang wheat areas were tested by using molecular markers of stripe rust-resistant genes Yr10 and 1BL/1RS translocation. 【Result】Yr10 was only detected in Xindong No. 17 and 1BL/1RS was detected in Xindong No. 24,Xindong No. 46,MJ307,HMJ555,1112,Xinzi No. 1,983-S,Xinmai 211 and Xinmai 23,which accounted for 19. 57% among the total testing materials. 【Conclusion】The distribution of Yr10 which demonstrates good resistance to CYR32 is low in Xinjiang wheat region cultivars,and Xindong No. 17 which may contain Yr10 has certain value in breeding disease resistance materials,which shows that the distribution frequency of 1BL/1RS translocation should be reduced when breeding new varieties.