新疆阿魏菇细菌性斑点病病原菌的鉴定

Identification of Pathogens Causing Bacterial Spot Disease on Pleurotus Ferulae(Pleurotus. Eryngii var. ferulae) in Xinjiang

【目的】研究新疆地区阿魏菇细菌性斑点病的病原菌。【方法】应用菌落特征、致病性测定、16S rDNA序列测定、序列同源性比较,及生理生化反应特征,对阿魏菇细菌性斑点病病原菌分离物进行鉴定。【结果】从阿魏菇子实体病害样品中分离到19个单菌落分离物,并对该19个分离物进行致病性检测,其中有8个分离物在健康阿魏菇子实体上产生了细菌性斑点病症状,通过16S rDNA序列比对、序列同源性比较和生理生化反应分析,该8个分离物均为假单胞杆菌(Pseudomonas spp.)。【结论】新疆地区阿魏菇细菌斑点病由假单胞杆菌(Pseudomonas spp.)引起。

[Objective] The objective of this study is to identify the pathogens that cause bacterial spot disease of Pleurotus ferulae in Xinjiang. [ Method] The causal agents were identified by colony characterization, pathogenicity test, 16S rDNA sequence analyses, sequence homology comparison, and biochemical characterization tests. [ Result] Nineteen bacterial isolates were isolated from diseased samples of Pleurotus ferulae. Through pathogenicity test, eight tested isolates caused typical symptoms of bacterial spot disease on the healthy fruiting bodies of Pleurotus ferulae. Based on pathogenicity test, and combined with the results of 16S rDNA sequence analyses, sequence homology comparison, biochemical and molecular characterization, eight isolates were identified as Pseudomonas spp. [ Conclusion] The bacterial spot disease on Pleurotus ferulae in Xinjiang is incited by Pseudomonas spp.