甜瓜“黄旦子”再生体系的建立

Establishment of Regeneration System for Cucumis melo L.cv.‘Huangdanzi’ of Melon

以甜瓜品种"黄旦子"的子叶为试材,采用组织培养的方法,研究了子叶日龄、不同浓度6-BA、IAA和头孢霉素对不定芽的影响。结果表明:子叶日龄对其不定芽的诱导具有较大影响,最佳诱导时间为子叶光照下培养2d,不定芽诱导率达80%;MS+6-BA 1.2mg·L^(-1)为最佳诱导培养基,诱导率达67%。此外,500mg·L^(-1)头孢霉素可作为脱菌和抑菌过程的最佳浓度,最佳卡那霉素浓度为25mg·L^(-1)。

Cucumis melon L. cv. ‘ Huangdanzi＇ cotyledon were used as test materials, using the method ot tissue culture,effects of the cotyledon day age, different concentrations of 6-BA,IAA and eefotaxime on adventitious buds were studies. The results showed that the cotyledon age played an important role in inducing adventitious buds,the optimal induction time was two days after cotyledon cultured under light, the regeneration rate was 80%. In addition, MS＋ 6-BA 1.2 mg · L-1 was the best induction medium, the regeneration rate was 67%. Furthermore,500 mg · L-1 cefotaxime was the best concentration that could be used to eradicate agrobacterium, 25 mg · L-1 kanamyein was the best screening concentration.