西芹鲜根六次醇层物化感物质的分离及其对黄瓜枯萎病菌的化感作用

Separation of Allelochemicals of the Sixth Chromatography Fractions of Ethylalcohol Extracts of Fresh Parsley Roots and Its Allelopathic Effects on Fusarium oxysporum f.sp.cucumberinum

以西芹鲜根为试材,经乙醇浸提后,采用注层析法、化感作用检测法和GC-MS检测法,研究了西芹鲜根乙醇浸提液经第5、6次层析分离获得各层析流分与黄瓜枯萎病菌共培养,并计算出化感效果筛选出最佳流分,最后通过GC-MS检测出6次醇层物最佳流分中的化感物质成分,从而达到西芹鲜根中化感物质成分的纯化目的。结果表明:各流分均对黄瓜枯萎病菌具有较强抑制作用,其化感效果与乙醇对照差异显著,第5次层析最佳流分为RE211018、RE29554、RE62359和RE68656,化感效果(相对于乙醇对照)依次为63.05%、70.84%、69.38%和59.16%;第6次层析最佳流分为RE2110186、RE295548、RE623597和RE686568,其化感效果(同上)分别提高至70.14%、71.38%、64.84%和60.63%。西芹鲜根第6次醇层物4个最佳流分中含有酚类、酯类、胺类和烷烃类共4类11种化感物质,分别为2,4-二叔丁基苯酚、8,11-十八碳二烯酸甲酯、芥酸乙酯、邻苯二甲酸二甲酯、邻苯二甲酸异丁酯、邻苯二甲酸丁基2-乙基己基酯、邻苯二甲酸丁基酯2-戊基酯、邻苯二甲酸二丁酯、(Z)-9-十八烯酸酰胺、二十七烷和3-乙基-5-(2-乙基丁基)十八烷。

Athylalcohol extracts of fresh parsley roots was used as the experimental material,and the methods has been applied with chromatography by a column with silicone coating,allelopaphy testing and GC-MS.We studied that ethylalcohol extracts of fresh parsley roots has been proceed with the fifth and sixth chromatography fractions and incorporated the different degree column fractions into PDA medium and co-cultured the plates with F.oxysporumf.sp.cucumberinum.The best fractions of ethylalcohol extracts of parsley fresh root were screened according to the allelopathic inhibition effect.At last,further allelochemicals of the sixth chromatography was identified by GC-MS.So as to achieve the purpose of identifing inhibitory allelochemicals released into the soil by parsley crop.The results showed that there were significant different allelopathic effect for the degree column fractions obtained from the fifth and sixth cycles of chromatography respectively on F.oxysporum f.sp.cucumberinum.The allelopathic effects of the fifth optimal fractions（RE211018,RE29554,RE62359 and RE68656）compared with the fifth cycle tomographic ethylalcohol（the control,ECK1）was 63.05%,70.84%,69.38%and 59.16%respectively.The allelopathic effects of the sixth optimal fractions（RE2110186,RE295548,RE623597 and RE686568）compared with the sixth cycle tomographic ethylalcohol（the control,ECK2）was70.14%,71.38%,64.84%and 60.63%respectively.There were 11 kinds of allelochemicals contained in Phenol,ester,amine and alkanes 4classes in the GC-MS analysis of the sixth chromatography fractions of ethylalcohol extracts of parsley fresh root.They were 2,4-bis（1,1-dimethylethyl）-phenol,8,11-octadecadienoic acid,methyl ester,Ethyl 13-docosenoate（ethyl erucate）,Dimethyl phthalate,1,2-benzenedicarboxylic acid,butyl2-methylpropyl ester,1,2-benzenedicarboxylic acid,butyl 2-ethylhexyl ester,phthalic acid,butyl 2-pentyl ester,dibutyl phthalate,9-Octadecenamide,（Z）-,heptacosane,3-ethyl-5-（2-ethylbutyl）-octadecane.