鲜菌草与灵芝菌丝发酵菌质多糖提取工艺及其体外抗氧化性

Extraction Technology of Fermentation Substance by Fresh JUNCAO and Ganoderma lucidum Polysaccharides and Antioxidant Activity in vitro

以鲜菌草与灵芝菌丝发酵得到的菌质为试材,采用单因素试验和正交实验对其多糖进行提取,研究了不同的料液比、提取时间、提取温度和提取次数对菌质多糖提取率的影响,获得最优化的提取条件;用Fenton体系和邻苯三酚自氧化体系研究了菌质多糖对羟自由基和超氧自由基清除率的影响。结果表明:影响鲜菌草与灵芝菌丝发酵菌质多糖提取得率的主次因素分别为提取温度>料液比>提取次数>提取时间;最佳提取工艺条件为料液比1∶30g·mL^(-1)、温度100℃、提取时间4h、提取次数3次,以此最佳组合提取鲜菌草与灵芝菌丝发酵菌质多糖时,其得率为4.88%。鲜菌草与灵芝菌丝发酵菌质多糖对羟基自由基和超氧阴离子自由基有明显的清除作用,并且随着多糖浓度的增加,清除能力增强,表明鲜菌草与灵芝菌丝发酵菌质多糖对羟基自由基和超氧阴离子自由基的清除能力与多糖浓度有明显的量效关系。通过相关方程可以得到鲜菌草与灵芝菌丝发酵菌质多糖清除羟基自由基的EC_(50)值为0.461mg·mL^(-1),清除超氧阴离子自由基的EC_(50)值为0.864mg·mL^(-1)。

Fermentation substance of fresh JUNCAO and Ganoderma lucidum were used as test material. By single factor test and orthogonal test,the effect of different solid-liquid ratio, extraction time, extraction temperature and extraction times on the yield of polysaccharide extraction were studied, in order to obtain extraction condition optimization. Then by the Fenton system and the pyrogallol autoxidation system, the scavenging rate of fermentation substance of fresh JUNCAO and C-anoderma lucidum to polysaccharide hydroxyl radical and superoxide radical were studied. The results showed that the primary and secondary factors affecting the extraction yield of polysaccharides were extraction temperature〉solid liquid ratio〉extraction times〉extraction time. The optimum extraction conditions were the solid liquid ratio of 1 ： 30 g · mL-1 , temperature 100 ℃, extraction time 3 hours,4 times of extraction,and under the best combination the extraction of polysaccharide, the yield was 4. 88%. Fermentation substance polysaccharide had obvious scavenging effect on hydroxyl radical and superoxide anon radical, and with the increase of the polysaccharide density, the clearance ability enhanced. It showed that the density of the fermentation substance polysaccharide had an evident does-effect relationship with the clearance ability of hydroxyl radical and superoxide anon radical. According to the relevant equations, fermentation substance polysaccharide EC50 was 0. 461 mg · mL-1, and fermentation substance polysaccharide EC50 was 0. 864 mg · mL-1.