空心柴胡不同部位中柴胡皂苷a、d和总黄酮的含量比较

Content Comparison of Saikosaponin a,d and Total Flavonoids in Different Parts of Hollow Bupleurumin

目的:比较空心柴胡不同部位中柴胡皂苷a、d和总黄酮的含量,为临床合理使用药用部位提供参考。方法:柴胡皂苷a、d含量测定采用HPLC法:色谱柱Inertsil C_(18)柱(250 mm×4.6 mm,5μm);流动相:乙腈-水(40∶60),流速:1.0 ml·min^(-1);检测波长:210 nm,柱温:40℃,进样量:10μl。总黄酮的测定采用比色法:检测波长为500 nm。结果:柴胡皂苷a、柴胡皂苷d和柴胡总黄酮分别在0.21~1.26 mg·ml^(-1)(r=0.999 6)、0.25~1.51 mg·ml^(-1)(r=0.999 7)和4.00~25.00μg·ml^(-1)(r=0.999 6)范围内与峰面积呈良好的线性关系;平均回收率分别为99.27%(RSD=2.15%,n=6)、99.4%(RSD=2.14%,n=6)和99.03%(RSD=1.34%,n=6)。空心柴胡根中柴胡皂苷a、d含量分别为0.31%、0.50%,其他部位含量较低;总黄酮在花中含量高达8.48%,在叶、茎、根中依次降低。结论:空心柴胡地上部分富含黄酮类化合物,根中柴胡皂苷含量较高,临床使用带根全草比较合理。

Objective： To compare the contents of saikosaponin a, d and total flavonoids in different parts of Hollow bupleurum to provide reference for the clinical use of medicinal parts. Methods： The determination was performed on a SHIMADZU Inertsil C18 （250 mm ×4.6 mm,5 μm） column. The mobile phase consisted of acetonitrile and water with the ratio of 40 ： 60, and the flow rate was 1.0 ml·min^-1. The detection wavelength was 210nm, the column temperature was 40℃, and the injection volume was 10 μl. A colorimetric method was used to detect total flavonoids with the detection wavelength of 500 nm. Results ： The calibration curve of Saikosaponin a, d and total flavonoids showed a good linear relationship respectively over the range of 0.21-1.26 mg·ml^-1 （ r = 0. 999 6 ） ,0.25-1.51 mg·ml^-1 （r =0.9997） and 4.00-25.00μg·ml^-1 （r =0.999 6）. The average recovery was 99.27% （RSD =2.15%, n=6） ,99.4% （RSD =2.14% ,n =6）and 99.03% （RSD = 1.34% ,n =6）, respectively. The content of saikosaponin a and d respectively was 0.31% and 0.50% in the root, while that in the other parts was low. The content of total flavonoids was as high as 8.48% in flowers, and that in leaves, stem and root reduced in turn. Conclusion： The aerial parts of Hollow bupleurum are rich in flavonoids, and the content of saikosaponin in root is higher, therefore, the whole plant with root is more reasonable in the clinical use.