生川乌配伍白蔹、白及反药组合在大鼠血中移行成分的研究

Absorbed constituents in rat plasma after administered crude Aconiti Radix in compatibility with Ampelopsis Radix or Bletillae Rhizoma

目的鉴定生川乌配伍白蔹、白及的入血成分,比较配伍前后血中移行成分的变化特征。方法大鼠ig给予生川乌配伍白蔹、白及提取物,收集血浆样品并采用UPLC-QTOF/MS法鉴定入血成分,由半定量分析比较配伍前后血中移行成分的变化。结果检测配伍组20个成分,均为来自于生川乌的原形成分,配伍组中双酯型生物碱的相对质量分数均显著降低,单酯型生物碱中苯甲酰乌头原碱的相对质量分数显著降低,醇胺型生物碱中宋果灵的相对质量分数显著降低,其他没有显著性变化。结论生川乌配伍白蔹、白及能够改变其血中移行成分的体内过程。

Objective To identify the absorbed constituents in rat plasma after administered crude Aconiti Radix in compatibility with Ampelopsis Radix or Bletillae Rhizoma, and to compare changes of the absorbed constituents in plasma before and after compatibility. Methods Rats were given extract of crude Aconiti Radixin compatibility with Ampelopsis Radix or Bletillae Rhizoma by intragastric administration, and the plasma samples were collected and analyzed by UPLC-QTOF/MS method. Changes of the migrating components in rat plasma before and after compatibility were compared by semi-quantitative analysis. Results Twenty kinds of components were detected in rat plasma, which were all prototype components from Aconiti Radix. After compatibility, the contents of diester-type alkaloids were significantly decreased, and the contents of benzoylaconitine （monoester-type alkaloid） and songorine （alcohol amine-type alkaloid） were significantly decreased, but there were no significant changes in other components.Conclusion Compatibility of Aconiti Radix with Ampelopsis Radixor Bletillae Rhizomacould change thein vivo process of absorbed constituents in plasma.