摘要
Functional nucleic acids(FNAs)-based biosensors have shown great potential in heavy metal ions detection due to their low-cost and easy to operate merits. However, in most FNAs based fluorescence probes, the ingenious designs of double-labeled(fluorophore and quencher group) DNA sequence, not only bring the annoyance of organic synthesis, but also restrict its use as a robust biosensor in practical duties. In this paper, we design a simple AIEgens functional nucleic acids(AFNAs) probe which consists of only fluorogen but no quencher group. With the help of duplex-specific nuclease(DSN) enzyme based target recycling, high fluorescence signal and superior sensitivity towards Hg^(2+) are achieved. This robust assay allows for sensitive and selective detection of Hg^(2+) in real water samples and mapping of intracellular Hg^(2+), without double-labeling of oligonucleotide with a dye-quencher pair, nor the multiple assay steps.
Functional nucleic acids （FNAs）-based biosensors have shown great potential in heavy metal ions detection due to their low-cost and easy to operate merits. However, in most FNAs based fluorescence probes, the ingenious designs of double-labeled （fluorophore and quencher group） DNA sequence, not only bring the annoyance of organic synthesis, but also restrict its use as a robust biosensor in practical duties. In this paper, we design a simple AIEgens functional nucleic acids （AFNAs） probe which consists of only fluorogen but no quencher group. With the help of duplex-specific nuclease （DSN） enzyme based target recycling, high fluorescence signal and superior sensitivity towards Hg2＋are achieved. This robust assay allows for sensitive and selective detection of Hg2＋ in real water samples and mapping of intracellular Hg2＋, without double-labeling of oligonucleotide with a dye-quencher pair, nor the multiple assay steps.
基金
supported by the National Natural Science Foundation of China(21525523,21574048,21375042,21405054)
the National Basic Research Program of China(2015CB932600,2013CB933000)
the Special Fund for Strategic New Indus-try Development of Shenzhen,China(JCYJ20150616144425376)
1000 Young Talent(to Fan Xia)
