摘要
以酶法水解冷榨花生粕蛋白质粉制备得到的花生多肽液(<3 000 u)为原料,以氯化亚铁为金属螯合剂,在单因素试验基础上,利用Box-Behnken响应面优化技术对多肽-亚铁螯合条件进行优化分析并获得最优螯合工艺参数,即多肽-亚铁质量比为4.31:1(g/g),螯合温度为25.4 C,螯合时间28.5 min和螯合pH 7.5,在此优化条件下,多肽-亚铁螯合率为(85.68±1.27)%(n=3),与模型预测值89.653 1%接近,偏差为4.64%。花生粕蛋白多肽-亚铁螯合物经紫外光谱和红外光谱分析发现,Fe^(2+)与多肽中的NH_2^+以及COO-形成共价配位键并形成稳定的共轭结构,是一种新型有机金属螯合物。
Peanut polypeptide liquid ( 〈 3 000 u) that prepared by enzymatic hydrolysis of peanut protein power from cold -pressed peanut meal (CPPM) was raw metarial and the ferrous chloride was metal chelator. The optimal chelate technological parameters was obtained after the the optimized analysis to the polypeptide - ferrous chelator by u- sing Box -Behnken response surface optimization technique based on single factor experiment, ie. , polypeptide -fer- rous mass ratio was 4.31 : 1 ( g/g), chelating temperature was 25.4 ℃, chelating time was 28.5 rain and chelating pH was 7.5. Under the optimization condition, the polypeptide - ferrous chelation rate was (85.68 ± 1.27) % ( n = 3 ), which was well agreement with the model predicted value ( 89.653 1% ), and the deviation between the confirmative re- sult and the predicted result was 4.64%. Through ultraviolet spectroscopy (UV) and infrared spectrometer(FT- IR) a- nalysis, peanut meal albumen polypeptide - ferrous chelate was found, the Fe2 + and NH2 +in the polypeptide and COO - form covalence coordinate bond and form stable conjugated structure, which was a new -type organic metal -chelator.
作者
李玉珍
肖怀秋
赵谋明
林亲录
Li Yuzhen Xiao Huaiqiu Zhao Mouming Lin Qinlu(Pharmaceutical and Bioengineering School, Hunan Chemical Industry and Vocational Technology College , Zhuzhou 412004 College of Light Industry and Food science, South China University of Technology, Guangzhou 510640 College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004)
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2017年第4期64-69,共6页
Journal of the Chinese Cereals and Oils Association
基金
湖南省高校科研项目(12C1049)
湖南化工职业技术学院院级项目(HNHY2015002)
2016年湖南教育厅科学研究项目(16C0550)
关键词
冷榨花生粕
多肽
多肽-亚铁螯合物
响应面优化
cold - pressed peanut meal ( CPPM ), polypeptide, polypeptide - ferrous chelator ( PFC ), response surface optimization.