赤芝中羊毛脂烷型三萜类化合物及其α-葡萄糖苷酶抑制活性研究

Lanostane triterpenoids of Ganoderma lucidum and their inhibitory effect on α-glucosidase

目的研究赤芝Ganoderma lucidum的干燥子实体乙醇提取物的化学成分及其活性。方法运用硅胶柱色谱、中压液相色谱、HPLC等色谱技术对赤芝95%乙醇提取物进行系统分离,并结合UV、HR-ESI-MS、ESI-MS、1D-NMR、2D-NMR等波谱学手段对所得到的化合物进行结构鉴定;考察化合物的α-葡萄糖苷酶抑制活性。结果从赤芝95%乙醇提取物中分离得到11个三萜类化合物,分别鉴定为灵芝醇A(1)、灵芝酸H(2)、灵芝酸AM1(3)、灵芝酸D(4)、灵芝酸D甲酯(5)、ganolucidic acid E(6)、11α-hydroxy-3,7-dioxo-5α-lanosta-8,24(E)-dien-26-oic acid(7)、灵芝烯酸D(8)、赤芝酸A(9)、赤芝酸F甲酯(10)、赤芝酸B(11)。结论化合物1为新化合物,化合物1和5显示了一定的α-葡萄糖苷酶抑制作用。

Objective To study the chemical constituents from the dried fruiting body of Ganoderma lucidum and its activities. Methods The compounds were isolated and purified from the 95% ethanol extract of G. lucidum by silica gel CC, MPLC, HPLC, and so on. Their structures were identified by extensive spectroscopic analysis. The compounds were evaluated for their inhibitory effects against α-glucosidase compared with those of the positive control acarbose. Results A total of 11 lanostane triterpenoids were isolated from the 95% ethanol extract of G. lucidum and identified as ganoderanol A（1）, ganoderic acid H（2）, ganoderic acid AM1（3）, ganoderic acid D（4）, methyl ganoderate D（5）, ganolucidic acid E（6）, 11α-hydroxy-3,7-dioxo-5α-lanosta-8,24（E）-dien-26-oic acid（7）, ganoderenic acid D（8）, lucidenic acid A（9）, methyl lucidenate F（10）, and lucidenic acid B（11）. Conclusion Among them, compound 1 is a new compound. Additionally, compounds 1 and 5 show moderate inhibitory effect on α-glucosidase.