高效液相色谱切换波长法同时测定大鼠血浆中绿原酸和栀子苷的浓度

Simultaneous determination of chlorogenic acid and geniposide in rat plasma by HPLC wave-length switching method

目的:建立同时测定大鼠血浆中绿原酸和栀子苷浓度的方法。方法:以甲醇为溶剂,采用蛋白沉淀法处理血浆,采用高效液相色谱(HPLC)法测定药物浓度。色谱柱为大连依利特C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%甲酸(14∶86),内标为紫丁香苷。内标、绿原酸、栀子苷的检测波长分别为266 nm(0~5.9 min)、328 nm(6.0~7.9 min)和234 nm(8.0~12min)。结果:绿原酸和栀子苷分别在1.625~104.0,1.925~123.2μg·mL^(-1)内线性关系良好,回收率分别在92.25%~99.36%和91.96%~98.57%范围内,RSD均低于6%。结论:该方法简便、准确,可用于同时测定绿原酸和栀子苷的血药浓度。

OBJECTIVE To establish a HPLC method for simultaneous determination of chlorogenic acid and geniposide in rat plasma.METHODS The plasma was deproteinated by adding methanol prior to HPLC determination.The analysis was performed on a Hypersil ODSC18 column（250 mm×4.6 mm,5μm）.The mobile phase was acetonitrile-0.1%formic acid（14∶86）with syringin as the internal standard.The detection wavelengths of syringin,chlorogenic acid and geniposide were set at 266 nm（0-5.9 min）,328 nm（6.0-7.9 min）and 234 nm（8.0-12 min）,respectively.RESULTS The method achieved a good linearity in the range of 1.625-104.0μg·mL^-1 for chlorogenic acid,and 1.925-123.2μg·mL^-1 for geniposide.The recoveries of chlorogenic acid and geniposide were within 92.25%-99.36% and 91.96%-98.57%,respectively.CONCLUSION This method is convenient and accurate,and can be used to determine chlorogenic acid and geniposide in rat plasma.