摘要
目的:建立同时测定大鼠血浆中绿原酸和栀子苷浓度的方法。方法:以甲醇为溶剂,采用蛋白沉淀法处理血浆,采用高效液相色谱(HPLC)法测定药物浓度。色谱柱为大连依利特C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%甲酸(14∶86),内标为紫丁香苷。内标、绿原酸、栀子苷的检测波长分别为266 nm(0~5.9 min)、328 nm(6.0~7.9 min)和234 nm(8.0~12min)。结果:绿原酸和栀子苷分别在1.625~104.0,1.925~123.2μg·mL^(-1)内线性关系良好,回收率分别在92.25%~99.36%和91.96%~98.57%范围内,RSD均低于6%。结论:该方法简便、准确,可用于同时测定绿原酸和栀子苷的血药浓度。
OBJECTIVE To establish a HPLC method for simultaneous determination of chlorogenic acid and geniposide in rat plasma.METHODS The plasma was deproteinated by adding methanol prior to HPLC determination.The analysis was performed on a Hypersil ODSC18 column(250 mm×4.6 mm,5μm).The mobile phase was acetonitrile-0.1%formic acid(14∶86)with syringin as the internal standard.The detection wavelengths of syringin,chlorogenic acid and geniposide were set at 266 nm(0-5.9 min),328 nm(6.0-7.9 min)and 234 nm(8.0-12 min),respectively.RESULTS The method achieved a good linearity in the range of 1.625-104.0μg·mL^-1 for chlorogenic acid,and 1.925-123.2μg·mL^-1 for geniposide.The recoveries of chlorogenic acid and geniposide were within 92.25%-99.36% and 91.96%-98.57%,respectively.CONCLUSION This method is convenient and accurate,and can be used to determine chlorogenic acid and geniposide in rat plasma.
作者
李菲
田景振
刘振中
LI Fei TIAN Jing-zhen LIU Zhen zhong(Department of Pharmacy, Shangdong University of Traditional Chinese Medicine, Shangdong Jinan 250355, China Department of Pharmacy, Taishan Medical University, Shangdong Taian 271016,China)
出处
《中国医院药学杂志》
CAS
北大核心
2017年第8期687-689,共3页
Chinese Journal of Hospital Pharmacy
基金
国家科技重大新药创制专项(编号:2014ZX09509001)