地黄多糖对K562细胞增殖抑制作用及bcr/abl融合基因表达的影响

Effects of Rehmannia glutinosa polysaccharides on K562 cell proliferation and bcr/abl fusion gene expression

目的:研究地黄多糖(RPS)对K562细胞bcr/abl融合基因表达和细胞增殖的影响。方法:不同浓度RPS作用于K562细胞,MTS法检测其对细胞增殖的影响,Real-time PCR检测bcr/abl融合基因mRNA表达变化,Western blotting检测bcr/abl融合基因表达的P210蛋白变化。结果:RPS能够抑制K562细胞增殖,用400μg·mL-1 RPS作用12 h,细胞增殖抑制率为35.16%,随着RPS浓度增加抑制作用明显增强(P<0.05);用200μg·mL-1 RPS作用24 h,K562细胞内bcr/abl融合基因mRNA相对表达量为0.26,P210蛋白相对含量为0.35。RPS使K562细胞内P210蛋白及bcr/abl融合基因mRNA表达量呈时间和剂量依赖性下降。结论:RPS可能通过下调bcr/abl融合基因mRNA及P210蛋白表达对K562细胞增殖产生抑制作用。

OBJECTIVE To investigate the effects of Rehmannia glutinosa polysaccharides（RPS）on the proliferation and bcr/abl expression of K562 cells.METHODS Human leukemia cell line K562 was cultured in vitro.After K562 cells were incubated with RPS at different concentrations（0,50,100 and 200μg·mL^-1）for 12 h,48 h and 72 h,K562 cell proliferation was assayed by MTS,bcr/abl fusion gene expression was tested by real time PCR and P210 bcr/abl protein by Western blot.RESULTS K562 cell proliferation was significantly inhibited by RPS.Inhibition rate was 35.16% with 400μg·mL^-1 RPS at 12 h.After RPS was added,the growth inhibition was remarkable.The relative expression of bcr/abl mRNAvs.control was 0.26.Relative expression of P210 protein vs.control was 0.35 with 200μg·mL^-1 RPS at 24 h.The inhibition effect showed a dose and time dependent fashion.CONCLUSION RPS can inhibit the proliferation of K562 cells in vitro,which is probably correlated with down regulation of bcr/abl fusion gene at mRNA and protein levels.