长链非编码RNA PANDAR对乳腺癌细胞增殖和侵袭的影响

Effect of long noncoding RNA PANDAR on the proliferation and invasion of breast cancer cells

目的观察长链非编码RNA（lncRNA） PANDAR对乳腺癌细胞增殖和侵袭的影响。方法采用慢病毒表达系统，构建稳定过表达及干扰PANDAR的MCF-7细胞；通过细胞计数试剂盒（CCK-8）实验和Transwell实验分别检测乳腺癌细胞的增殖和侵袭能力。结果感染PANDAR过表达慢病毒的MCF-7，PANDAR的表达水平为20.05±1.08，显著高于对照组细胞（P＝0.000）；而感染干扰PANDAR慢病毒的MCF-7细胞，PANDAR的表达水平为0.16±0.02，显著低于对照组细胞（P＝0.008）。24、48、72 h，PANDAR过表达组的细胞增殖活性（0.489±0.067、0.945±0.025、1.236±0.072），均高于对照组细胞（0.385±0.024、0.576±0.059、0.737±0.041），差异有统计学意义（P＝0.045、0.024、0.012）；PANDAR干扰组的细胞增殖活性（0.292±0.055、0.371±0.041、0.455±0.068），均低于对照组细胞，差异有统计学意义（P＝0.036、0.030、0.014）。PANDAR过表达组侵袭细胞数显著高于对照组[（48.37±13.08）个比（25.15±6.75）个，P＝0.011]。而PANDAR干扰组侵袭细胞数显著低于对照组[（23.35±2.96）个比（50.13±11.87）个，P＝0.015]。结论PANDAR促进乳腺癌细胞的增殖和侵袭。

Objective To investigate the effect of long noncoding RNA （lncRNA） PANDAR on the proliferation and invasion of breast cancer cells. Methods We constructed the stable MCF -7 cells with overexpression or knockdown of PANDAR by using lentivirus system and detected the cell proliferation and invasion by cell counting kit - 8 （ CCK - 8） assay and matrigel Transwell assay. Results The relative expression of PANDAR in MCF - 7 cells with overexpression of PANDAR was 20.05 ± 1.08, higher than in the control group （P = 0. 000）, and that in MCF -7 cells with knockdown of PANDAR was 0. 16 ±0. 02, lower than in the control group （ P =- 0. 008）. The cell proliferation activity of PANDAR overexpres- sion group （0. 489 ± 0. 067, 0. 945 ± 0. 025, 1. 236 ± 0. 072 ） at 24, 48 and 72 h, were higher than these in control group （0. 385 ± 0. 024, 0. 576±0. 059, 0. 737 ± 0. 041 ; P = 0. 045, P = 0. 024, P = 0. 012, respectively）. The cell proliferation activity of PANDAR knockdown group （0. 292 ± 0. 055, 0. 371 ± 0. 041, 0. 455± 0. 068 ） at 24, 48 and 72 h, were significantly lower than these in control group （ P = 0. 036, P =0. 030, P = 0. 014）, respectively. The amount of invasion cells in PANDAR overexpression group were significantly more than control group （48.37±13. 08 vs. 25. 15 ±6. 75; P=0. 011）. While the amount of invasion cells in PANDAR knockdown group were significantly less than control group （23.35 ± 2. 96 vs. 50. 13 ± 11.87; P = 0. 015 ）. Conclusion PANDAR promotes cell proliferation and invasion of breast caner cells.