摘要
目的观察保胆手术对胆结石豚鼠模型免疫功能及胃肠功能的影响。方法 取经致石食谱喂养8周后胆结石豚鼠模型30只为研究对象,随机分为胆囊切除组和保胆手术组各15只,胆囊切除组行胆囊切除术,保胆手术组行保胆手术,术后4周处死豚鼠,比较两组免疫功能、小肠与结肠推进功能、绒毛高度、绒毛宽度、黏膜厚度等指标。结果 保胆手术组豚鼠小肠组织CD3+T细胞、CD40+B细胞、免疫球蛋白IgA阳性细胞计数均明显高于胆囊切除组[(9.85±1.02)比(7.56±0.84)、(9.15±1.12)比(7.24±0.80)、(9.24±1.05)比(7.36±0.83)个/视野;t=6.712、5.375、5.400,P=0.004、0.011、0.001);小肠与结肠推进速度明显低于胆囊切除组[(36.12±4.25)%比(44.02±5.32)%,(5.21±0.64)%比(7.54±0.78)%;t=4.494、8.944,P=0.021、0.013];空肠与回肠绒毛高度、绒毛宽度、黏膜厚度均明显大于胆囊切除术组[(265.02±34.25)比(240.05±30.24),(72.05±8.54)比(62.25±7.36),(346.21±44.12)比(314.65±40.26),(236.12±32.52)比(210.36±23.02),(76.14±8.65)比(65.47±7.58),(424.12±42.21)比(385.42±35.32) μm;t=2.117、3.367、2.046、2.504、3.593、2.723,P=0.015、0.023、0.020、0.033、0.026、0.022]。结论 保胆结石术有助于保护胆结石豚鼠肠道免疫功能,减少肠黏膜组织受损,促进肠道吸收。
Objective To study the effect of gallbladder surgery on immune function and gastrointestinal function of guinea pigs with gallstones model, and provide reference for individualized treatment of gallstones.Methods Thirty guinea pigs with gallstones model were randomly divided into gallbladder surgery group and cholecystectomy group, given gallbladder surgery and cholecystectomy respectively. Four weeks after surgery, the animals were killed. The immune function, small intestine and colon propulsion function, villus height, villus width, and mucosal thickness were compared between two groups.
Results In the gallbladder surgery group, the cells of CD3+ T cells, CD40+ B cells, immunoglobulin IgA in the small intestine tissue were significantly more than those in the cholecystectomy group (9.85±1.02 vs. 7.56±0.84, 9.15±1.12 vs. 7.24±0.80, and 9.24±1.05 vs. 7.36±0.83; t=6.712, 5.375, 5.400, P=0.004, 0.011, 0.001). Small intestine and colon advancing speed in the gallbladder surgery group was significantly slower than in the cholecystectomy group [(36.12±4.25)% vs. (44.02±5.32)%, and (5.21±0.64)% vs. (7.54±0.78)%; t=4.494, 8.944, P=0.021, 0.013]. The jejunum and intestinal villus height, villus width, and mucosal thickness were significantly greater in the gallbladder surgery group than in the cholecystectomy group [(265.02±34.25) vs. (240.05±30.24), (72.05±8.54) vs. (62.25±7.36), (346.21±44.12) vs. (314.65±40.26), (236.12±32.52) vs. (210.36±23.02), (76.14±8.65) vs. (65.47±7.58) and (424.12±42.21) vs. (385.42±35.32) μm; t=2.117, 3.367, 2.046, 2.504, 3.593 and 2.723, P=0.015, 0.023, 0.020, 0.033, 0.026, 0.022].Conclusion Gallbladder surgery helps to protect intestinal immune function of guinea pig with gallstones, reduce intestinal mucosal damage and promote intestinal absorption.
出处
《中华实验外科杂志》
CSCD
北大核心
2017年第4期605-607,共3页
Chinese Journal of Experimental Surgery
基金
河南省高校科技创新人才支持项目(14HASTIT030)
关键词
胆结石
豚鼠
保胆结石术
胆囊切除术
免疫功能
黏膜屏障功能
Gallstones
Guinea pig
Gallbladder surgery
Cholecystectomy
Immune function
Mucous barrier function