人Runt相关转录因子3在由结直肠息肉进展至结直肠癌过程中表达的变化

The expression of human Runt - related transcription factor 3 in the progression from colorectal polyps to cancer

目的 观察人Runt相关转录因子3（RUNX3）基因在由结直肠息肉进展至结直肠癌过程中表达水平的变化。 方法 本研究分为5组，分别为结直肠癌30例、与其配对的正常结直肠黏膜组织30例（距肿瘤〉5 cm）、管状腺瘤30例、绒毛状腺瘤20例及混合性腺瘤30例。应用免疫组织化学及实时荧光定量聚合酶链反应（FQ-PCR）方法观察RUNX3在结直肠腺瘤及结直肠癌组织中的表达变化。结果 免疫组织化学结果显示RUNX3在正常结直肠黏膜组、结直肠腺瘤组、结直肠癌组中表达之间差异有统计学意义（P＝0.015），阳性表达率分别为96.7%、73.8%、46.7%；FQ-PCR显示RUNX3在正常结直肠黏膜、结直肠管状腺瘤、结直肠混合性腺瘤、结直肠绒毛状腺瘤及结直肠癌组织中依次降低，其表达量分别为33.930 7±8.5709、16.4591±3.3837、7.901 2±2.081 6、3.714 6±1.710 9、0.871 5±0.523 1。SNK多重比较结果显示任两组间总体均数差异有统计学意义（F＝47.859，P＝0.021）。 结论 RUNX3在结直肠癌的发生过程中起重要作用。

Objective To explore the expression of human runt-related transcription factor 3 （RUNX3） in the progression from colorectal polyps to cancer. Methods In this study, five groups of specimens were researched： primary colorectal cancer group （n=30）, matched normal colorectal tissues group （5 cm away from the tumour, n=30）, tubular adenoma group （n=30）, villous adenoma group （n=20）, and adenoma mixed group （n=30）. The protein and mRNA levels of RUNX3 were detected by immunohistochemistry and real-time fluorescent quantitative polymerase chain reaction （FQ-PCR） respectively.Results The positive expression rate of RUNX3 protein in normal colorectal mucosa, colorectal adenoma and colorectal carcinoma tissues was 96.7%, 73.8% and 46.7% respectively, with significant differentces among them （P=0.015）. FQ-PCR showed that the expression of RUNX3 mRNA in normal colorectal mucosa, tubular adenoma, mixed pattern of adenoma, villous adenoma and colorectal carcinoma was 33.930 7±8.570 9, 16.459 1±3.383 7, 7.901 2±2.081 6, 3.714 6±1.710 9, and 0.871 5±0.523 1 respectively, F=47.859, P=0.021.Conclusion These results suggested RUNX3 may play a role in the development of colorectal cancer.