长链非编码RNA CAI2在神经胶质瘤组织的表达及临床意义

Expression and clinical significance of long non - coding RNA - CAI2 in glioma

目的 探讨长链非编码RNA（lncRNA） CAI2在神经胶质瘤中的表达及临床意义。 方法 收集手术切除的神经胶质瘤及癌旁脑组织标本各65例，利用实时定量聚合酶链反应（Real-time PCR）方法检测CAI2的mRNA表达水平，分析其与临床病理特征及生存期的相关性。 结果 CAI2在神经胶质瘤组织中的表达水平为2.691±1.845，明显高于正常脑组织（1.079±0.851，P＝0.009）；Ⅲ～Ⅳ级患者的表达水平（3.219±1.883）明显高于Ⅰ～Ⅱ级患者（1.926±1.304，P＝0.008），CAI2高表达率也与肿瘤世界卫生组织（WHO）分级明显相关（P＝0.018），高表达的患者总生存率明显低于低表达者（Log-rank＝8.573，P＝0.003），CAI2高表达是神经胶质瘤患者预后不良的独立风险因子[风险比（HR）＝1.707，P＝0.044]。结论 lncRNA CAI2可以做为神经胶质瘤的预后评估因子。

Objective To study the expression and clinical significance of long non-coding RNA （lncRNA）-CAI2 in gliama.Methods Sixty-five specimens of the glioma and normal brain tissues undergoing surgical operation were selected. The CAI2 expression was detected by real-time quantitative polymerase chain reaction（Real-time PCR）. The correlation of the CAI2 with clinicopathological parameters and survival of patients was analyzed statistically. Results The expression level of CAI2 in glioma （2.691±1.845） was obviously higher than that in normal brain tissues （1.079±0.851, P=0.009）, and that of CAI2 in glioma of grade Ⅲ-Ⅳ （3.219±1.883） was obviously higher than that of grade I-II （1.926±1.304, P=0.008）. Additionally, high expression rate of CAI2 was related with the World Health Organization （WHO） grade （P=0.018）. Moreover, overall survival rate of glioma with CAI2 high-expression was significantly lower than that with CAI2 low expression （Log-rank=8.573, P=0.003）, and CAI2 high-expression was an independent risk factor of poor prognosis for glioma patients [hazard ratio （HR）=1.707, P=0.044]. Conclusion.LncRNA-CAI2 may be used as a potential marker to evaluate the glioma prognosis.