新型碳源驯化SRB生长特性及处理酸性矿山废水研究

Growth Characteristics of SRB Domesticated with New Organic Carbon Sources and Its Application in AMD Treatment

利用生活污水、鸡粪和锯末质量比为80∶7∶3混合物的发酵液作为碳源驯化硫酸盐还原菌(SRB),研究了在该新型碳源条件下SRB的生长规律、还原SO_4^(2-)反应动力学及对酸性矿山废水(AMD)中重金属离子和SO_4^(2-)的去除效果。结果表明,SRB生长适应期在0~1 d,对数期在1~6 d,稳定期在6~9 d,衰亡期在9 d后;SRB对SO_4^(2-)有很好的去除效果,去除率可达98.2%;且当F/M(SO_4^(2-)/污泥浓度)值>7 mg/g时处于对数生长期,当3 mg/g≤F/M值≤7 mg/g时处于生长稳定期,当F/M值<3 mg/g时进入衰亡期;SRB的最大比生长速率为1.2 d^(-1);SRB还原SO_4^(2-)符合经典Monod方程;SRB对AMD中Fe^(2+)、Mn^(2+)、Cu^(2+)、Zn^(2+)、SO_4^(2-)的去除率分别达到93%、81%、65%、93%、89%,出水p H值接近中性。以该新型碳源作为SRB的营养物质处理AMD可实现以废治废,为实际应用提供了理论参考。

The fermentation liquor of mixture was used as organic carbon sources to domesticate SRB. The mixture were composed of domestic sewage, chicken feces and saw dust on the scale of 80 ： 7 ： 3. The growth rhythm of SRB, the reaction kinetics of sulfate reduction and the treatment effect of heavy metal ions and sulfate in acid mine drainage were studied. The results showed that SRB grew through four stages ： slow period （0 - 1 d）, logarithmic phase （ 1 - 6 d ）, stable period （ 6 - 9 d） and decline period （ after 9 d） ; SRB had a good effect on removing sulfate, and the removal rate was 98.2% ; when F/M （SO]- to sludge ratio） value was more than 7 mg/g, between 3 mg/g and 7 mg/g, and less than 3 mg/g, SRB lay in logarithmic phase, stable period and decline period, respectively; the maximum specific growth rate of SRB was 1.2 d^-1 ; the sulfate reaction kinetics of SRB conformed to the Monod equation; the removal rates of Fe^2＋ , Mn^2＋ , Cu^2＋ , Zn^2＋ and SO4……2- in acid mine drainage could reach up to 93% , 81% , 65% , 93% and 89% , respectively. And pH was close to neutral. Treating acid mine drainage by SRB with the new organic carbon sources can achieve the goal of using waste to treat waste.