摘要
病毒诱导基因沉默(virus-induced gene silencing,VIGS)是一种研究基因功能的反向遗传工具,该技术具有操作简单快速、周期短、高通量、无需遗传转化、成本低等优点,在基因探寻和功能鉴定上广泛应用。着重围绕上述病毒诱导基因沉默技术在研究茄科(Solanaceae)植物的相关功能基因在植物次生代谢、生长发育、生物和非生物胁迫几个方面的应用进行了综述和展望。
Virus-induced gene silencing (VIGS) is areverse-genetics technology for studying gene function in plants and it's widely used in the gene identification and function validation owing to simple operating, short cycle, high-throughput, transformation-free and low cost. This paper reviews and then prospects mainly the application and development of the VIGS technology in research of functional genes related with plant secondary metabolism, growth and development, biotic and abiotic stress in Solanaceae Nants.
出处
《辣椒杂志》
2017年第1期1-12,共12页
Journal of China Capsicum
基金
国家自然科学基金项目(31572124)
广东省公益研究与能力建设专项(2015B020202009
2014B020202005)
广州市科创委项目(201508030021)
关键词
茄科植物
VIGS
功能基因组学
次生代谢
生长发育
生物和非生物胁迫
Solanaceae plants
VIGS
functional genomics
secondary metabolism
growth and development
biotic and abiotic stress