棉花GhbHLH基因的克隆与表达分析

Molecular Cloning and Expression Analysis of GhbHLH from Upland Cotton

bHLH(basic helix-loop-helix,bHLH)家族基因在植物生长发育和非生物胁迫应答中发挥着重要的作用。本研究从前期构建的棉花cDNA文库中发掘得到一个新的GhbHLH基因,该基因开放阅读框全长600 bp,编码199个氨基酸,序列分析表明,该基因具有典型的helix-loop-helix结构域。荧光定量PCR表明,GhbHLH在棉花根、茎、叶和纤维中均有表达,并且能够受高盐、PEG、低温、ABA和Me JA等的诱导表达,亚细胞定位结果表明,GhbHLH在细胞核内表达。推测该基因可能作为调控因子在棉花非生物胁迫应答中发挥作用。

bHLH （basic helix-loop-helix, bHLH） gene family transcription factors play important roles in plant growth and abiotic stress response. In this study, we cloned a new bHLH gene from a normolized cDNA library, which was named GhbHLH. GhbHLH contained an open reading frame （ORF） of 600 bp, which encoded a protein with 199 amino acids, and contained an Helix-Loop-Helix conserved domain. Quantitative RT-PCR analysis showed that GhbHLH expressed in root, stem, leaf and fiber, and was induced by salt, PEG, cold, ABA and MeJA, subcellular localization analysis showed that GhbHLH-GFP fusion protein was distributed specifically in cell nucleus. Our data indicated GhbHLH may play important role in the stress response in cotton.