Sysmex XE-5000血细胞分析仪对异型淋巴细胞和嗜碱性粒细胞的报警提示分析

Analysis of the Sysmex XE 5000 blood cell analyzer for the analysis of abnormal lymphocytes and basophils

目的 探讨Sysmex XE-5000血细胞分析仪在异型淋巴细胞与嗜碱性粒细胞报警提示功能的价值.方法 采集198份Sysmex XE-5000血细胞分析仪同时提示异型淋巴细胞与嗜碱性粒细胞增多的标本,915份该仪器单独提示异型淋巴细胞增多标本.行显微镜血涂片复查,以显微镜下检出异型淋巴细胞为金标准核实报警提示是否准确.结果 以显微镜复检结果为金标准,同时提示异型淋巴细胞与嗜碱性粒细胞增多的标本经复检后,嗜碱性粒细胞阳性数值≥1.8%,在正常范围,异型淋巴细胞的阳性率为65.2%（129/198）,但仪器单独出现异型淋巴细胞报警提示的标本经显微镜复查后异型淋巴细胞的阳性率为72.4%（663/915）,前者低于后者,差异有统计学意义（χ^2=4.521,P〈0.05）.结论 Sysmex XE-5000血细胞分析仪对异型淋巴细胞与嗜碱性粒细胞同时出现报警提示时,两种检测水平存在干扰,需结合显微镜复查,利于提高检测的准确率,降低漏诊或误诊率,为临床治疗提供可靠数据.

Objective To explore the value of Sysmex XE 5000 blood cell analyzer in alarm of atypical lymphocytes and basophil.Methods 198 samples of Sysmex XE 5000 blood cell analyzer and suggested that atypical lymphocytes and basophils, 915 copies of the instrument tip shaped specimens.Single lymphocyte increased blood smear microscopy for examination under a microscope to detect atypical lymphocytes as the gold standard and to verify the alarm not exactly.Results The microscopic examination results as the gold standard, and suggested that atypical lymphocytes and basophilia specimens after reinspection, eosinophilic granulocyte alkaline or positive value of 1.8%,in the normal range, the positive rate of atypical lymphocytes （129/198） was 65.2%,but the instrument alone atypical lymphocytes alarm specimens the positive rate of atypical lymphocytes after review of 72.4% microscope （663/915）,the former was lower than the latter with significant difference （χ^2=4.521,P〈0.05）.Conclusion Sysmex XE 5000 blood cell analyzer at the same time of atypical lymphocyte and basophil alarm, two detection levels of interference, combined with microscope examination can improve detection accuracy, reduce the misdiagnosis rate, and provide reliable data for clinical treatment.