从调控TGF-β/Smads通路途径探讨龙葵防治肝癌的机理

Mechanism of Solanum nigrum in the prevention and treatment of liver cancer from the regulation of TGF-β/Smads pathway

目的:探讨龙葵提取物对HepG2细胞增殖、侵袭力和细胞凋亡的影响,阐明龙葵防治肝癌细胞凋亡的机理。方法:选取肝癌患者50例为研究对象,采集其肝癌标本并将处于对数生长期的HepG2细胞分别加入合适浓度的龙葵提取物(龙葵组)、磷酸缓冲盐溶液(PBS组)、转化生长因子-β抑制剂Decorin(TGF-β组),采用TUNEI法及RT-PCR法检测凋亡细胞及HepG2细胞中TGF-β、SMAD3、SMAD4基因表达。结果:在抑制HepG2细胞增殖、降低侵袭力以及促进细胞凋亡比较上,龙葵组效果最佳,PBS组最差,TGF-β组居中,且均P<0.05;三组TGF-β、SMAD3、SMAD4基因表达比较有统计学差异(均P<0.05)。结论:龙葵提取物可通过调控TGF-β/Smads通路来抑制肿瘤细胞增殖和侵袭力、促进细胞凋亡,对于肝癌的治疗具有重要意义。

Objective：To investigate the effects of solanum nigrum extract on HepG2 cell proliferation,invasion and apoptosis,to clarify the mechanism of solanum nigrum in the prevention and treatment of liver cancer cells apoptosis.Methods：50cases of patients with liver cancer as the research object,collected liver cancer specimens and HepG2 cells in the exponential growth phase were added to solanum nigrum extract of the appropriate concentration（solanum nigrum group）,phosphate buffered saline（PBS group）,transforming growth factor beta inhibitors（TGF-βgroup）,TUNEI method and RT-PCR method were used to detect the expression of TGF-β,SMAD3 and SMAD4in apoptotic cells and HepG2 cells.Results：In inhibiting HepG2 cell proliferation,reducing the invasiveness and promoting cell apoptosis,the effects of solanum nigrum group was the best,the worst in PBS group,TGF-βgroup was center,P〈0.05.Compared the expression of TGF-β,SMAD3 and SMAD4genes in the three groups,P〈0.05.Conclusion：Solanum nigrum extract can regulate TGF-β/Smads pathway to inhibit proliferation and invasion of tumor cells,promote cell apoptosis,plays an important role in the treatment of liver cancer.