葛根通络颗粒对偏头痛大鼠模型的作用及机制研究

Effects of the Gegen Tongluo granules on the migraine model of rats and the mechanism

目的:观察葛根通络颗粒(GG)对硝酸甘油致大鼠偏头痛模型的作用,探讨其缓解偏头痛的机理。方法:采用皮下注射硝酸甘油的方法建立偏头痛大鼠模型,观察3h内大鼠前肢挠头次数,测定血清一氧化氮(NO)含量及一氧化氮合酶(NOS)活性,脑组织5-羟色胺(5-HT)、去甲肾上腺素(NA)水平,并检查血液流变学指标。结果:葛根通络颗粒3.0、6.0g·kg^(-1)减少模型大鼠的前肢挠头次数,降低偏头痛大鼠血清中NO的含量,增加脑组织内5-HT含量,6.0g·kg-1降低全血粘度。结论:葛根通络颗粒有一定防治偏头痛的功效,其机制可能与调节体内5-HT、NO水平以及改善机体的血液流变学有关。

Objective：To observe the effect of Gegen Tongluo sranules（GG）on the model of migraine induced by nitroglycerin in rats,and to explore the mechanism of relieving migraine.Methods：The rats model was established by subcutaneous injection of nitroglycerin.The symptoms of scratching head with forepaws were observed for three hours,nitric oxide（NO）content and nitric oxide synthase（NOS）activity in serum,5-serotonin（5-HT）and norepinephrine（NA）levels in brain,and the hemorheology were determined.Results：The number of scratching head with forepaws,the level of NO in serum were reduced significantly,serotonin levels in the rat brain were improved obviously at GG 3and 6.0g·kg-1,and also,the blood viscosity was decreased at GG 6.0g kg-1.Conclusion：Gegen Tongluo granules have a certain effects on migraine,and its mechanism may be related to regulate 5-HT and NO levels and improve the hemorheology.