翻译后修饰蛋白质组学分离方法的研究进展

Recent advances in separation methods for post-translational modification proteomics

蛋白质翻译后修饰(PTMs)是调节细胞内生理活动的重要途径。该文总结了近年来PTMs蛋白质组学相关的分离方法,包括反相(RP)色谱法、离子交换(IEX)色谱法、亲水相互作用色谱(HILIC)法、多孔石墨化碳(PGC)色谱法、毛细管电泳(CE)法及分子筛色谱(SEC)法等。这些新方法为磷酸化、乙酰化、糖基化等PTM肽段或蛋白质的鉴定提供了更高的分离度和灵敏度。此外,该文也介绍了蛋白质领域其他重要分离方法的研究进展,这些方法可能被进一步应用于PTMs蛋白质组学的研究中。

Post-translational modifications （PTMs） are the key control of many cellular processes. Current progresses of separation methods for PTMs proteomics,including reversed phase （RP） chromatography,ion exchange （IEX） chromatography,hydrophilic interaction chromatography （HILIC）,porous graphitic carbon （PGC） chromatography,capillary electrophoresis （CE） and size-exclusion chromatography （SEC） are reviewed. The methods for the identification of PTMs have higher resolution and sensitivity. In addition,some other important separation methods for proteomics study are introduced,and they may be applied in the study of PTMs with further optimization in the future.