5-甲基硫代腺苷对结肠癌Hct116细胞增殖的影响

Role of methylthioadenosine in cell proliferation of colon cancer Hct116 cells

目的研究5-甲基硫代腺苷(MTA)对结肠癌Hct116细胞增殖的影响及其相关机制。方法采用MTT比色法及软琼脂克隆形成试验分析MTA对结肠癌Hct116细胞增殖的影响,采用Realtime PCR的方法分析RNA聚合酶Ⅲ依赖基因(tRNAs、5S rRNA)及TFⅢB亚单位TBP、Bdp1和Brf1基因mRNA水平的变化,并采用Western Blot的方法分析了TFⅢB亚单位Bdp1蛋白水平的变化。结果 MTA对结肠癌Hct116细胞具有明显的增殖抑制作用,在这一过程中,RNA聚合酶Ⅲ依赖基因(tRNAs、5S rRNA)的mRNA水平明显下调;同时TFⅢB亚单位Bdp1在mRNA水平和蛋白水平也出现了明显下调。结论 MTA对结肠癌Hct116细胞具有明显增殖抑制作用;RNA聚合酶Ⅲ依赖基因转录的下调、TFⅢB亚单位Bdp1蛋白的下调可能在这一抗肿瘤过程中起到重要作用。

Objective To study the role and mechanism of methylthioadenosine in cell proliferation of colon cancer Hctl 16 cells. Meth-ods MTT assay and anchorage-independent growth were used to detect the effects of methylthioadenosine on proliferation in Hctl 16 cells;the level of RNA Pol Ⅲ-dependent （tRNAs,5SrRNA） and the subunits of TFⅢB （TBP,Bdp1 and Bif l） transcription were de-tected with the method of Realtime PCR;the level of subunit of TFⅢB （Bdp1） protein was detected with the method of Western Blot. Results Methylthioadenosine could significantly inhibitthe proliferation of Hctl 16 cells; RNA Pol Ⅲ-dependent （tRNAs,5S rRNA） transcription was downregulated significantly ; mRNA and protein of the Bdp1 were also downregulated significantly in this course. Con-clusions Methylthioadenosine could inhibit significantly the proliferation ofHctll6 cells and downregulate RNA Pol Ⅲ-dependent （tRNAs,5S rRNA） transcription and protein of the subunit of the TFⅢB Bdp1,which might play a major role in the treatment of cancer.