乌药水提液对腹泻型肠易激综合征模型大鼠Ghrelin、MTL、SP、Sec水平的影响

Effect of Water Extract of Lindera Aggregata on Ghrelin, MTL, SP and Sec Levels in Rats With Diarrhea-Predominant Irritable Bowel Syndrome

目的探讨乌药水提液对腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome,IBS-D)大鼠血清生长激素释放肽(Ghrelin)、胃动素(Motilin,MTL)、P物质(P substance,SP)、促胰液素(Secretin,Sec)水平的影响。方法将60只大鼠随机分成6组,采用单只孤养1周+番泻叶煎剂灌胃2周+慢性束缚应激1周造模法建立IBS-D大鼠模型,分别给予乌药水提液低、中、高剂量,匹维溴铵灌胃干预。采用酶联免疫吸附法(ELISA)检测各组大鼠血清Ghrelin、MTL、SP、Sec含量。结果与空白对照组比较,模型对照组SP、Ghrelin、MTL水平显著升高,Sec水平降低(P<0.01),提示造模成功;与模型对照组比较,乌药水提液低、中、高剂量组以及阳性对照组血清SP、MTL水平均明显降低,Sec水平均显著升高(P<0.01),乌药水提液低、中、高剂量组以及阳性对照组对Ghrelin影响无显著差异(P>0.05)。结论乌药水提液可能通过降低大鼠血清SP、MTL水平来改善IBS-D大鼠腹痛、腹泻症状。

Objective To investigate the water extracts of Lindera aggregata on Ghrelin, MTL, SP and Sec levels in rats with diarrhea -predominant irritable bowel syndrome. Methods 60 rats were randomly divided into 6 groups. Using a single solitary 1 weeks, gavage of sennoside for 2 weeks and chronic restraint 1 weeks to establish the rat model of IBS -D. The model rats were administrated with low, medium, high dose of water extracts of Lindera aggregata, and pinaverium bro-mide. The levels of SP, MTL, Sec and Ghrelin in serum of rats were measured by using enzyme linked immunosorbent assay （ELISA）. Results Compared with the blank control group, SP,Ghrelin and MTL levels significantly increased in the model control group,Sec was lower than the control group （P〈0.01）. Compared with the model group,the plasma levels of SP,MTL in the low, medium, high dose water extracts groups and pinaverium bromide group decreased, the Sec levels significantly increased,with statistical significance （P〈0.01）,while Ghrelin in the above four groups had no statistical significance （P〉0.05）. Conclusion The water extracts of Lindera aggregata could improve the abdominal pain and diarrhea of IBS-D model rats by reducing the plasma levels of SP and MTL.