菜芙蓉总黄酮纯化及其体内抗氧化性

Purification of total flavonoids of Abelmoschus manihot(L.)Medic and its antioxidant properties in vivo

为研究菜芙蓉总黄酮(TFA)的纯化条件及体内抗氧化性,采用AB-8大孔树脂静态吸附-洗脱工艺,控制纯化条件为上样液质量浓度0.1g/mL,pH值为3.5,解吸液乙醇体积分数为70%,解吸液体积与树脂质量比为20∶1(mL/g)。在最优纯化条件下,黄酮的质量分数提高到48.50%;与正常对照组相比,实验组小鼠器官指数无显著差异(P>0.05);小鼠血清和肝组织中MDA含量显著低于正常对照组(P<0.05),而CAT,GSH-Px和SOD活性均高于正常对照组且具有明显的量效关系。实验结果表明,TFA具有良好的体内抗氧化效果。

In order to optimize the purification conditions and the antioxidant properties in vivo of total flavonoids from A b e l-moschus manihot （L. ） Medic, AB-8 macroporous resin is used for static adorption and elution. The optimum purification condi-tions are as follows： Sample solution concerntration of 0. 1 g /mL , pH 3. 5, 70% ethanol as eluant, 20 ： l （mL/g ） of eluant volum to resin weight ratio. The results in the optimum purification conditions show that the purity of TFA reaches 48. 50%. There are no significant difference in indexes of mice organs between experimental groups and normal control group （P〉 0. 05）. MDA content exhibits a significant decrease both in serum and liver of mice （P〈C0. 05）. While the level of CAT, GSH- Px and SOD activities in serum and liver are higher in experimental groups than those in normal control group showing dose- response relationship. These results indicate that TFA has good antioxidant properties in vivo.