miR-100抑制乳腺癌细胞迁移的作用和机制

The action and mechanisms of miR-100 on decreasing migration ability of breast cancer cells

目的:探究miR-100对乳腺癌细胞株MDA-MB-231迁移能力的调节与机制。方法:Real timePCR检测人正常乳腺上皮细胞MCF-10A和乳腺癌细胞株MDA-MB-231中miR-100的基础表达水平。应用脂质体法将miR-100 mimic及阴性对照分别转染乳腺癌细胞株MDA-MB-231,通过real time-PCR检测转染后miR-100的表达水平,细胞划痕实验检测过表达miR-100对MDA-MB-231细胞迁移能力的影响,Western blot方法检测slug、snail和E-cadherin等EMT蛋白表达水平的变化。结果:miR-100在乳腺癌细胞株MDA-MB-231中的表达明显低于人正常乳腺上皮细胞MCF-10A。转染miR-100 mimic的乳腺癌细胞株MDA-MB-231的miR-100表达水平明显增高,细胞划痕实验显示过表达miR-100的MDAMB-231细胞划痕愈合速度明显减慢。过表达miR-100的MDA-MB-231细胞E-cadherin蛋白表达水平明显增加,而slug和snail蛋白表达水平明显降低。结论:miR-100抑制乳腺癌细胞株MDA-MB-231的迁移能力与其上调E-cadherin,下调slug、snail蛋白表达,抑制EMT有关。

ObjectiveTo investigate the effects and mechanisms of miR - 100 on the metastasis of breast cancer cell line MDA - MB -231. Methods： The expression of miR - 100 in human normal breast epithelial cells MCF - 10A and breast cancer cell line MDA - MB -231 were analyzed by real - time PCR. The expression of miR - 100 was detected by real time - PCR after transfecting miR -100 mimic or the negative control into MDA - MB - 231 breast cancer cell line. The cell migration ability of MDA - MB - 231 cells after transfection were detected by cell scratch test. The expression of slug,snail and E - cadherin were detected by Western blot. Results： The expression of miR - 100 in breast cancer cell line MDA - MB -231 was significantly lower than that in normal human breast epithelial cell line MCF - 10A. The expression of miR - 100 was significantly increased, cell scratches healing was significantly decreased, and the expression of E - cadherin protein was increased, while the expression of slug and snail protein was decreased in the MDA - MB -231 cells transfected with miR - 100 mimic. Conclusion：miR - 100 decreased the mi-gration ability of breast cancer cells by inhibiting the EMT of the cells.