非小细胞肺癌胸腔积液与配对肿瘤组织中EGFR基因突变检测的比较

The study of detection EGFR gene mutation in non-small cell lung cancer tissue and matched pleural effusion

目的:探讨非小细胞肺癌胸腔积液与配对肿瘤组织标本中EGFR基因突变检测结果的一致性,评价胸腔积液标本检测EGFR基因突变的应用价值。方法:收集非小细胞肺癌患者胸腔积液与配对肿瘤组织样本72例,采用ARMS方法,检测样本中EGFR基因第18~21外显子突变情况。结果:细胞学样本和组织学样本中EGFR基因突变阳性率分别为48.61%和51.39%,两者差异无统计学意义(P>0.05),二者一致率为92.11%,不一致率为7.89%。结论:二者的一致率较高,恶性胸水可以作为无法获得肿瘤组织的晚期非小细胞肺癌EGFR基因检测的有效样本。

ObjectiveTo investigate the consistency of EGFR gene mutations in pleural effusion of non - small cell lung cancer and matched tumor tissue samples,and to evaluate the application value of EGFR gene mutation in pleural effusion specimens. Methods ： 72 cases of non - small cell lung cancer patients with pleural effusion and matched tumor tissue samples were collected,using ARMS method to detect exon 18 ？21 mutations in EGFR gene. Results：The positive rates of EGFR gene mutation in cytology and histology samples were 48. 61% and 51.39% Re-spectively, and the difference was not statistically significant （P 〉0. 05）. The consistency rate of the two groups was 92. 11% ,and the inconsistent rate was 7. 89%. Conclusion ： The consistency of the two patients was so high that the malignant pleural effusion can be used as a substitute for the detection of EGFR gene in patients with advanced non - small cell lung cancer.