胶体金快速试纸条法测定小麦及其制品中呕吐毒素

Rapid determination of deoxynivalenol in wheat and its products by colloidal gold stripes

用胶体金快速试纸条法测定小麦及其制品中呕吐毒素,方法快速,定量结果较为准确,回收率在86.67%~120%。与高效液相法进行比较,结果表明这2种方法对呕吐毒素的检测没有显著的差异,其回收率略高于高效液相法,但变异系数大。在较低浓度(<1 500μg/kg)时,快速法与高效液相法有一定的线性关系Y=1.077 3X+83.786(Y为胶体金快速法测得值,X为高效液相法测得值,R^2=0.963 7)。

Determination of deoxynivalenol （ DON ） in wheat and its products by colloidal gold strips was very fast and accurate. Its recovery rate was between 86.67% ？120%. Compared with the HPLC method, the results showed that there was no significant difference between the two methods in the detection of DON . The recovery rate was a little higher than HPLC . But the coefficient of variation was bigger. At low concentrations （ 〈1 500μg/kg） , the rapid method had a linear relationship with the standard method： Y = 1.0773X ＋83.786 （Y represent the result by rapid method, X represent by HPLC method, R^2 =0.963 7）.