三叶青石油醚萃取部位的化学成分研究

Study on the chemical constituents of the petroleum ether fraction from Tetrastigma hemsleyanum

目的研究三叶青石油醚萃取部位的化学成分。方法运用硅胶柱色谱、凝胶柱色谱和制备HPLC等色谱分离方法对三叶青石油醚萃取部位化学成分进行分离纯化,用质谱法和核磁共振波谱法等波谱学方法鉴定化合物的结构。结果从三叶青石油醚萃取部位分离得到5个化合物,分别鉴定为:9-羟基-10,12-十八碳二烯酸(1),(4R,5R)-4-羟基-5-异丙基-2-甲基环己-2-烯酮(2),(4S,5R)-4-羟基-5-异丙基-2-甲基环己-2-烯酮(3),(3R,4R,6S)-3,6-二羟基-1-薄荷烯(4),肉桂酸(5)。结论化合物1~5均为首次从崖爬藤属植物中分离得到。

Objective To investigate the chemical constituents of the petroleum ether fraction fromTetrastigma hemsleyanum.Methods The chemical constituents of the petroleum ether fraction were separated and purified fromTetrastigma hemsleyanum by column chromatography,such as silica gels,Sephadex LH-20 and semi-preparative HPLC.The structures were identified on the basis of spectroscopic analysis.Results Five compounds were isolated and their structures were identified as（10E,12E）-9-hydroxyoctadeca-10,12-dienoic acid（1）,（4R,5R）-4-hydroxy-5-isopropyl-2-methylcyclohex-2-enone（2）,（4S,5R）-4-hydroxy-5-isopropyl-2-methylcyclohex-2-enone（3）,（3R,4R,6S）-3,6-dihydroxy-1-menthene（4）,and cinnamic acid（5）.Conclusion Compounds 1-5 were abtained from the genus Tetrastigmafor the first time.