姜黄素对创面愈合作用的影响及其机制的初步探讨

Effect of curcumin on wound healing and its mechanism

目的观察姜黄素（Curcumin，Cur）对大鼠皮肤创面愈合作用的影响及其机制。方法取30只雄性SD大鼠，用6miD．打孔器在每只大鼠背部制作4个直径为6mm大小创面模型。每只大鼠的每个创面每天施加不同浓度姜黄素（30、15、0ms／mL）及PBS干预创面的愈合。在术后第1、3、7、10、14d随机取6只大鼠计算各创面愈合速率，采用免疫组织化学法检测各组中不同时间点各创面中血管内皮生长因子（vascular endothlial growth factor，VEGF）、碱性成纤维细胞生长因子（basic fibroblast growthfactor，bFGF）表达量，采用免疫荧光法检测各组中不同时间点各创面中M1型及M2型巨噬细胞含量。结果溶媒剂对创面愈合未见明显影响；与对照组相比，Cur15组及Cur30组创面愈合明显增快；Cur15组及Cur30组术后第7天和第10天创面VEGF免疫组化平均光密度数值均多于相同时间点Cur0组及PBS组（P〈0．05）；Cur15组术后第3、7和10天及Cur30组术后第7天和第10天bFGF免疫组化平均光密度数值均多于相同时间点CurO组及PBS组（P〈0．05）；Cur15组及Cur30组术后第3和7天创面CD68与诱导型一氧化氮合酶（inducible nitric oxide sythase，iNOS）双阳性细胞百分比均少于相同时间点CurO组及PBS组（P〈0．05）；Cur15组术后第3和7天及Cur30组术后第3、7、10和14天创面CD68与精氨酸酶1（arginase—1，Arg-1）双阳性细胞百分比均多于相同时间点Cur0组及PBS组（P〈0．05）。结论Cur能够明显促进创面愈合，其机制可能与Cur对M1与M2型巨噬细胞的极化调节有关；Cur浓度为15mg／mL时创面愈合速率最快。

Objective To observe the effect of curcumin on wound healing in a rat model and the mecha- nisms involved. Methods Thirty male Sprague Dawley rats were used in the study, and four 6 mm diameter size wound were made by puncher on the back of rats. Curcumin of different concentrations （30.15 and 0 mg/mL） and PBS were used to treat wound of rats everyday,respectively. On postinjury day1,3,7,10 and 14, respectively ,6 rats of injury group weres randomized chosen to measure the size of wound area. The expression of（ vascular endothlial growth factor） VEGF and （ basic fibroblast growth factor） bFGF in the wound tissue was observed with immunohistochemical staining. The content of M1 and M2 in the wound tissue was observed with immunofluorescence staining. Result The solvent had no effect on wound healing. Compared with control group, the wounds of group Curl5 and Cur30 healed significantly earlier. The results of wound VEGF mean optical density in group Curl5 and Cur30 were higher than these in group Cur0 and PBS on days 7 and 10 after operation （P 〈0.05）. The results of bFGF immunohistochemical average optical density in group Curl5 on days 3,7and 10 after operation and group Cur30 on days 7and 10 after operation were more than those in group Cur0 and PBS at the same time （ P 〈0. 05 ）. The percentage of wound CD68 and inducible nitric oxide synthase （iNOS） double positive cells was less than that in group CurO and PBS on days 3 and 7 after operation （ P 〈 0. 05 ）. The percentage of wound CD68 and（ arginase-1 ,Arg-1 ）double positive cells in group Cur15 and group Cur 30 respectively on days 3 ,7 and 3,7,10,14 after operation was higher than that in group Cur0 and PBS （P 〈0. 05）. Conclusions Curcumin could promote polarization of macrophages from M1 to M2 phenotype and exert protective effects on skin wound healing in rats.