血清Dickkopf1蛋白水平对原发性肝癌的诊断价值

The Diagnostic Value of Serum DKK1 Protein Level in the Primary Liver Cancer

目的:探索血清Dickkopf1(DKK1)水平对原发性肝癌(PLC)的诊断价值。方法:将我院收治的50例PLC患者、60例良性肝病患者、50例健康体检者及50例其它恶性肿瘤患者分别组成PLC组、良性肝病组、健康对照组及其他肿瘤组,采用ELISA定量检测各组血清DKK1蛋白的浓度,分析和比较其与α-fetoprotein(AFP)单独或联合诊断PLC的效能。结果:PLC组血清DKK1水平(838.96±104.14 ng/L)明显高于良性肝病组(322.61±25.44 ng/L)及健康对照组(213.03±25.70 ng/L),差异有统计学意义(P<0.05)。AFP、DKK1单独鉴别诊断PLC和良性肝病的灵敏度分别为66%、46%,特异度分别为81.7%、96.7%,一致率分别为71.8%、73.6%;两者联合诊断的灵敏度、特异度及一致率分别为84%、78.3%、80.9%。AFP、DKK1联合诊断PLC的灵敏度和一致率均明显高于AFP、DKK1单独诊断。结论:血清DKK1在原发性肝癌中高表达,与原发性肝癌的发生密切相关,对PLC的早期诊断有一定参考价值,与AFP联合诊断可有效提高PLC的诊断效能。

Objective：To explore the diagnostic value of serum dickkopfl （DKK1） protein level for the primary liver cancer （PLC）.Methods：50 PLC patients,60 patients with benign liver disease,50 cases of healthy physical examination and 50 patients with the other malignant tumor in our hospital were divided into the PLC group,benign liver disease group,healthy control group and other tumor groups respectively.ELISA was used to quantitatively detect the concentration of serum DKK1 protein in each group,and the diagnostic efficiency of DKK1 and α-fetoprotein （AFP） alone or in combination for PLC were analyzed and compared.Results：The serum DKK1 level （838.96± 104.14 ng/L） in PLC group was significantly higher than that in the benign liver disease group （322.61± 25.44 ng/L） and the healthy control group （213.03＋ 25.70 ng/L）（P〈0.05）.The sensitivity of DKK1 and AFP alone in the differential diagnosis of PLC and benign liver disease were 66%,46% respectively,the specificity were 81.7%,96.7% respectively and the concordance rate were 71.8%,73.6% respectively.The sensitivity,specificity and consistency of DKK1 and AFP in combination in the diagnosis of PLC were 84%,78.3%,80.9%,respectively.The diagnostic sensitivity and consistency of AFP and DKK1 in combination for PLC were significantly higher than those ofAFP,DKK1 alone.Conclusions：Serum DKK1 was highly expressed in PLC,which was closely related to the occurrence of PLC.It might be useful for the early diagnosis of PLC.Combined diagnosis with AFP could effectively improve the diagnostic efficacy of PLC.