血浆miR-122表达与肝癌手术前后肝损伤的相关性

Correlation between Expression of Plasma miR-122 and Liver Injury before and after Liver Cancer Surgery

目的:探讨血浆中micro RNA-122(miR-122)表达与肝癌根治切除术前后肝损伤的相关性。方法:选取2015年1月-2016年1月在我院择期行肝癌根治切除术的肝癌患者36例,作为研究组,另选择同期健康体检者30例作为对照组。分别取研究组患者术前、术后第1 d、7 d及对照组入组时的清晨肘静脉血,采取荧光定量PCR(RT-PCR)技术检测血浆中miR-122表达水平,并检测两组血浆丙氨酸氨基转移酶(ALT)及转化生长因子-β1(TGF-β1)水平,分析手术前后研究组中各指标的相关性。结果:研究组术前血浆miR-122表达水平与血浆ALT、TGF-β1水平均高于对照组(P<0.05)。与术前比较,研究组术后1 d血浆miR-122表达水平与血浆ALT、TGF-β1水平均明显上升(P<0.05);且与术后1 d比较,肝癌患者术后7 d血浆miR-122、ALT、TGF-β1水平明显下降,且与术前比较差异有统计学意义(P<0.05)。肝癌患者术前、术后1 d、7 d血浆miR-122表达水平与血浆ALT、TGF-β1水平均呈正相关(均P<0.05)。结论:血浆miR-122与肝癌根治切除术前后肝功能损伤有关,可作为一种新型生物学指标。

Objective： To explore the correlation between expression of plasma micro RNA-122 （miR-122） and liver injury before and alter radical resection of liver cancer. Methods： Selected 36 patients who were underwent selective radical resection of liver cancer as the research group in our hospital from January 2015 to January 2016, in the same time selected 30 cases of healthy physical examination as the control group. Collected the early morning elbow vein blood of research group before operation and 1 d, 7 d after operation and the control group, the expression of plasma miR-122 in two groups were detected by Real-time PCR （RT-PCR） technique, and detected the levels of plasma alanine aminotransferase （ALT） and transforming growth factor-β1 （TGF-β1）, then analyzed the correlation between above indexes in research group before and alter operation. Results： The expression of plasma miR-122, plasma ALT and TGF-β1 in the research group before operation were higher than that in the control group （P〈0.05）; In research group, the expression of plasma miR-122, plasma ALT and TGF-β1 ld after operation were significantly increased compared with before operation （P〈0.05）, but they were decreased 7 d alter operation compared with 1 d alter operation, which was statistically different from before operation（P〈0.05）; The expression of plasma miR-122 in research group before operation and 1 d, 7 d alter operation were positively correlated with plasma ALT and TGF-β1 （P〈0.05）. Conclusion： Plasma miR-122 is related to liver injury before and alter radical resection of liver cancer, which could be a new biological index.