异氟烷对小鼠星型胶质细胞Sirt1和MAO-A基因表达的影响

Effect of Isoflurane on the Expression of Sirt1/MAO-A in Cultured Astrocytes

目的:探讨异氟烷对小鼠星形胶质细胞Sirt1和MAO-A基因表达的影响。方法:给予体外培养的新生小鼠原代星形胶质细胞不同浓度异氟烷处理,实验分为对照组和异氟烷处理组(0.5ISO、1.0ISO、1.5ISO),其中异氟烷组细胞分别给予0.5 MAC、1.0MAC和1.5 MAC三个浓度的异氟烷处理2小时,对照组给予O_2处理2小时,然后提取细胞RNA和蛋白检测Sirt1和MAO-A的mRNA和蛋白表达变化。结果:与对照组相比,异氟烷下调小鼠星形胶质细胞的Sirt1和MAO-A mRNA和蛋白水平,异氟烷浓度越大下调越明显。结论:异氟烷可抑制小鼠星形胶质细胞的Sirt1和MAO-A基因表达。

Objective：To explore the effect of isoflurane on the expression of Sirtl/MAO-A of Astrocytes.Methods：Cultured astrocytes from newborn mice were divided into control and isoflurane groups：control group was treated with O2 for 2 hours and isoflurane treatment groups （0.5ISO,1.0ISO,1.5ISO） were treated with 0.5 MAC,1.0 MAC and 1.5 MAC of isoflurane for 2 hours respectively.Then total RNA and protein was extracted from cells and the mRNA and protein levels of Sirtl and MAO-A were measured by Real-time PCR and Western blot respectively.Results：（1） Compared with the control,Isoflurane significantly down-regulated the mRNA as well as protein levels of Sirtl and MAO-A in the cultured astrocytes;（2） The effect of isoflurane revealed a dose effect---compared with 0.5ISO group,cells treated with 1.0MAC and 1.5 MAC of isoflurane suffered more influence.Conclusion：Isoflurane exposure suppressed the expression of Sirt1/MAO-A of astrocytes from newborn mice brain,which is important for the isoflurane narcotherapy in depressive.