黑曲霉中疏水蛋白基因种类分析

Analysis of Hydrophobin Genes from Aspergillus niger

疏水蛋白对丝状真菌的形态有重要影响。现以基因序列的同源性、所编码氨基酸中8个半胱氨酸保守模式、氨基酸序列的水缘性图谱等指标对黑曲霉中可能的疏水蛋白基因进行分析。根据基因序列的同源性,将20个可能的黑曲霉疏水蛋白基因归为8个。这8个可能的疏水蛋白基因编码的蛋白质具有8个半胱氨酸保守模式和较高的基因序列同源性,在半胱氨酸保守模式上都属于I类疏水蛋白,但在水缘性图谱上其中只有2个疏水蛋白属于I类疏水蛋白。此外,这8个疏水蛋白的氨基酸序列同源性仅为0.5%。研究结果对黑曲霉中可能的疏水蛋白基因进行了梳理,将对研究疏水蛋白在丝状真菌形态控制中的作用具有重要意义。

Hydrophobins play an important role in the morphology engineering of filamentous fungi. To clar- ify hydrophobin genes of Aspergillus niger for further research, assumed hydrophobin genes were analyzed based on the cysteine pattern and hydropathy pattern of hydrophobin amino acid sequences. Eight genes were obtained from 20 assumed hydrophobin genes based on gene sequence similarity. The proteins coded by these eight genes had the 8 eysteines pattern which all belong to class I hydrophobin. While only two hy- drophobins had hydropathy pattern of class I, and the other 6 were considered as intermediate type of hy- drophobin according to their hydropathy patterns. Finally, the similarity of amino acid sequences of these 8 hydrophobins was 0.5%. The clarification of assumed hydrophobin genes builds a solid base for hydrophobin gene cloning and knockout to confirm their function in fungal spore aggregation.