摘要
目的探讨微小RNA(mi RNA)-21与mi RNA-155的表达水平及临床病理学特征与术后非小细胞肺癌(non-small cell lung cancer,NSCLC)患者预后的相关性。方法采用实时荧光定量聚合酶链反应检测152例NSCLC患者术后石蜡包埋组织标本中mi RNA-21、mi RNA-155的表达水平,采用单因素和多因素分析mi RNA-21与mi RNA-155表达水平、临床病理学特征与预后的相关性。结果单因素分析显示,与术后NSCLC患者预后相关的因素包括:年龄、肿瘤大小、mi RNA-21表达水平、TNM分期(P<0.05);多因素分析显示:年龄大(HR=1.027;95%CI:1.005~1.049)、TNM分期为Ⅱ期(HR=2.379;95%CI:1.385~4.086)和Ⅲ期(HR=2.521;95%CI:1.440~4.414)、癌组织中mi RNA-21表达水平升高(HR=1.149;95%CI:1.055~1.250)是影响术后NSCLC患者预后的独立危险因素。结论年龄大、TNM分期为Ⅱ期和Ⅲ期、癌组织中mi RNA-21表达水平升高是术后NSCLC患者预后的独立危险因素。
Objective To analyze the relationship between miRNA-21, miRNA-155, clinicopathological characteristics and prognosis among resected non-small cell lung cancer （NSCLC） patients. Method Analyzed the clinical and laboratory characteristics of 152 patients with NSCLC. Expression of miRNA-21 and miRNA-155 of the embedded tissue of the patients were detected by quantitative real-time polymerase chain reaction. Other clinicopathological characteristics were also collected. Result Univariate analysis identified significant prognostic clinical factors including age, stage, miRNA-21 expression, tumor size （P 〈 0.05）. In multivariate analysis, age （HR =1.027; 95%CI： 1.005- 1.049）, TNM stage： stage II （HR = 2.379; 95%CI： 1.385 - 4.086） and stage III （HR= 2.521; 95%CI： 1.440 - 4.414）, miRNA-21 expression increased in cancer tissues （HR ： 1.149; 95%CI： 1.055 -1.250） were independent prognostic factors. Conclusion Age, TNM stage： stage II and III, miRNA-21 increased in cancer tissues have the prognostic significance in postoperative NSCLC patients.
出处
《中国医学前沿杂志（电子版）》
2017年第3期13-16,共4页
Chinese Journal of the Frontiers of Medical Science(Electronic Version)
基金
北京市科委重大项目(D141100000214002)
