大黄素对功能性消化不良大鼠相关指标影响的实验研究

Experimental Study of Effect of Emodin on Correlated Indexes of FD Rats

目的:观察大黄素对功能性消化不良(FD)大鼠的促胃肠动力作用及对胃肠激素的影响。方法:雄性8周龄SD大鼠32只,随机分为正常组、模型组、大黄素组、西药组,每组8只。除正常组外,其他各组采用适度夹尾刺激法对大鼠进行造模。模型组及正常组大鼠灌胃蒸馏水,大黄素组和西药组分别给予大黄素、多潘立酮灌胃。7天后测量各组大鼠小肠碳末推进率,测定血清胃动素(MTL)和前列腺素E2(PGE2)水平。结果:与正常组比较,模型组、大黄素组、西药组大鼠体质量和MTL水平显著较低;模型组大鼠胃残留率显著升高,碳末推进率显著降低,差异均有统计学意义(P<0.05)。与模型组比较,大黄素组大鼠MTL和PGE2水平显著升高,胃残留率显著降低,碳末推进率显著升高;西药组胃残留率显著降低,碳末推进率显著升高,差异均有统计学意义(P<0.05)。与西药组比较,大黄素组大鼠MTL和PGE2水平升高的更明显,差异均有统计学意义(P<0.05,P<0.01)。结论:大黄素具有一定促胃肠动力作用,可能与提高胃动素有关,同时因其可提高PGE2水平,具有潜在的胃黏膜保护作用。

Objective：To observed the effect of emodin on gastrointestinal motility of functional dyspepsia（FD） rats and its effect on gastrointestinal hormone. Methods：Selected 32 eight-month-old healthy male rats, and divided them into the normal group,model group,emodin group,western medicine group with 8 cases in each randomly. Except for the normal group,the other groups were given proper tail stimulation method to establish models. The model group and the normal group were given distilled water by gavage, the emodin group and the western medicine group were given emodin and domperidone by gavage respectively. Detected the carbon powder propulsion of small intestinal,and levels of serum motilin（MTL） and prostaglandin E2（PGE2） of all groups seven days later. Results：Comparing with those of the normal group,body weight and MTL levels of the model group, the emodin group and the western medicine group were much lower. In the model group, as compared with those in the novmal group, gastric residual rate was increased significantly and carbon powder propulsion was decreased significantly（P〈0.05）. Comparing with those of the model group,MTL and PGE2 levels of the emodin group were increased significantly,gastric residual rate was decreased significantly and carbon powder propulsion was increased significontly（P〈0.05）. In the western medicine group, gastric residual rate was decreased significantly and carbon powder propulsion was increased significantly（P〈0.05）. Compared with that of the western medicine group,increase of MTL and PGE2 level of the emodin group was more significant（P〈0.05,P〈0.01）. Conclusion：Emodin has the function of gastrointestinal motility which may be related to the increase of motilin, and it has potential protective effect on gastric mucosa for it can increase the levels of PGE2.