前列舒宁胶囊对实验性前列腺炎大鼠的抗炎作用

Anti-Inflammatory Effect of Qianlie Shuning Capsules on Rats with Experimental Prostatitis

目的:观察前列舒宁胶囊对实验性前列腺炎大鼠的抗炎作用。方法:选取雄性Wistar大鼠120只,随机分为对照组、模型组、前列舒宁胶囊高、中、低各剂量组、前列通瘀胶囊组,分别用角叉菜胶和大肠埃希菌制造大鼠实验性非细菌性前列腺炎模型和细菌性前列腺炎模型。前列舒宁胶囊高剂量组、中剂量组、低剂量组分别灌胃1.5 g/(kg·d)、1.0 g/(kg·d)、0.5 g/(kg·d)的前列舒宁胶囊,前列通瘀胶囊组灌胃0.8 g/(kg·d)的前列通瘀胶囊,模型组和对照组灌胃蒸馏水,均为5.0 g/(kg·d),每天1次,连续9天。末次给药后1 h,将各组动物处死,摘取前列腺,取10μL前列腺按摩液,用白细胞稀释液稀释20倍,于显微镜下数白细胞数,另取1滴前列腺液涂片,镜下观察卵磷脂小体密度。结果:与对照组比较,模型组前列腺液中卵磷脂小体密度显著降低,白细胞总数显著升高,差异均有统计学意义(P<0.01),说明造模成功。与模型组比较,前列通瘀胶囊组、前列舒宁胶囊高剂量组、前列舒宁胶囊中剂量组前列腺液中卵磷脂小体密度显著升高,白细胞总数显著降低,差异均有统计学意义(P<0.05)。结论:前列舒宁胶囊对实验性前列腺炎大鼠有显著的抗炎作用。

Objective：To observe the anti-inflammatory effect of Qianlie Shuning capsules on rats with experimental prostatitis. Methods：One hundred and twenty male Wistar rats were selected and were divided into the control group,the model group,Qianlie Shuning capsules low,middle and high dosage groups and Qianlie Tongyu capsules group randomly.Carrageenan and escherichia coli were used to establish the rats model of experimental nonbacterial prostatitis and the rats model of experimental bacterial prostatitis respectively. Qianlie Shuning capsules high,middle and low dosage groups were respectively given Qianlie Shuning capsules of 1.5 g/（kg·d）,1.0 g/（kg·d） and 0.5 g/（kg·d） by gavage. Qianlie Tongyu capsules group was given 0.5 g/（kg·d） of Qianlie Tongyu capsules by gavage. The control group and the model group were both given5.0 g/（kg·d） of distilled water by gavage. All groups received medicine once a day and for nine days continuously. Rats in all groups were sacrificed after the last administration,and the prostates were removed. Expressed prostatic secretion of 10 μL was taken and diluted 20 times with the diluent of white blood cells for observation of white blood cells. One drop of prostatic fluid smear was additionally taken,and the density of lecithin corpuscles was observed under the microscope. Results：In the model group,the density of lecithin corpuscles in prostatic fluid was reduced evidently,and the content of white blood cells in prostatic fluid was increased obviously when compared with those in the control group（P〈0.01）, suggesting that the models were successful. Comparing with those in the model group, in Qianlie Tongyu capsules group, Qianlie Shuning capsules high dosage group and Qianlie Shuning capsules middle dosage group, the densities of lecithin corpuscles in prostatic fluid were all raised obviously,and the contents of white blood cells in prostatic fluid were all decreased significantly（P〈0.05）. Conclusion：Qianlie Shuning capsules have significant anti-inflammatory effect on rats with experimental prostatitis.