Sprouty2蛋白在胃癌组织中的表达及其意义

The expression and clinical significance of Sprouty2 in gastric cancer tissues

目的：探索Sprouty2（SPRY2）在胃癌及癌旁组织中的表达情况,分析其表达水平与临床病理因素及患者预后的相关性.方法：采用免疫组织化学的方法检测了121例胃腺癌标本中Sprouty2的表达.通过卡方检验评估了SPRY2表达与临床病理因素的相关性,并通过Kaplan-Merier法计算了SPRY2与总体生存率的关系,即SPRY2的预后意义.通过实时定量PCR的方法,我们检测了11对肿瘤组织和癌旁正常组织中SPRY2的mRNA水平并加以比较.结果：SPRY2在121例胃癌组织较低表达80例,较高表达41例,胃癌组织SPRY2表达水平较癌旁组织表达量显著降低.通过卡方检验证明SPRY2的表达与淋巴结转移（P〈0.001）和TNM分期明显相关（P=0.004）.Kaplan-Meier生存分析证明SPRY2低表达组与高表达组的病人存在显著的总体生存率的差异.SPRY2可以作为胃腺癌的一个预后标记物.结论：SPRY2高表达与胃癌病人不良预后明显相关,可作为胃癌预后的评价指标.

Objective： To explore the expression ot？.Sprouty2 （SPRY2） in gastric cancer and adjacent tumor tissue, and to analyze the correlation between SPRY2 expression and the clinicopathologic factors and prognostic value. Methods： We detected the expression of SPRY2 in 121 cases of gastric adenocarcinoma with irnmunohistochemistry, and further analyzed the correlation between SPRY2 and the clinicopathologic factors with Chi-square test. With Kaplan-Meier？method, we evaluated the relation between SRPY2 and overall survival rate. With real-time PCP,, we compared the mRNA levels of 11 pairs of gastric cancers and adjacent tumor tissues. Results： In the 121 cases of gastric adenocarcinoma, SPRY2 low expression accounted for 80 cases and SPRY2high expression？took up 41 cases. SPRY2 mRNA in gastric cancer was significantly lower than in adjacent tumor tissue. SPRY2 expression was demonstrated to be significantly associated with lymphatic invasion （P〈0.001） and TNM stage （P=0.004） . Survival analysis with Kaplan-Meier method proved that there was significant difference betweenSPRY2 high-expression group and low-expression group, indicating SPRY2 as a prognostic factor of gastric adenocarcinoma. Ooflclusiorl： SPRY2 high-expression was significantly associated with poorer prognosis of patients with gastric adenocarcinoma, and could be considered as a prognostic risk for gastric cancer.