化脓链球菌磷酸化蛋白组的分析

Analysis of Phosphoproteome of Streptococcus pyogenes

化脓链球菌是一种革兰氏阳性致病菌,严重威胁人类及家禽健康。运用溶液酶解的方法将化脓链球菌中的全蛋白消化成肽段,利用TiO_2富集化脓链球菌中的磷酸化肽段和Finnigan Surveyor HPLC系统串联LTQ-Orbitrap质谱对富集的磷酸化肽段进行分析。鉴定到167个磷酸化肽段,对应57个磷酸化蛋白。生物信息学分析显示这些蛋白主要涉及到细胞生物合成、细胞有机物及其衍生物代谢、信号传递、基因转录、生物应激等生理过程。通过STRING软件对这57个磷酸化蛋白进行相互作用网络分析,发现7个磷酸化蛋白相互作用网络。化脓链球菌的磷酸化蛋白组为深入解析化脓链球菌的毒力、耐药的发生发展机制提供理论基础,为开发新的抗菌药物提供新视角。

Streptococcus pyogenes is a Gram-positive pathogen, and it seriously threatens the health of human and fowls. The whole cell proteins in S. pyogenes were digested into peptide fragments by using trypsin solution. The phosphopeptides in S. pyo- genes were enriched through using a TiO2 enrichment kit, and the enriched phosphopeptides were analyzed by Finnigan Surveyor HPLC system and LTQ-Orbitrap mass spectrometer. A total of 167 phosphopeptides corresponding to 57 phosphorylated proteins were identified. The bioinformatics analysis revealed that these phosphoproteins mainly took part in the biosynthesis of cells, me- tabolism of cell organic matter and its derivatives, signal transduction, genetic transcription, biological stress and so on. The inter- active networks of these 57 phosphorylated proteins were analyzed by using STRING software, and 7 interactive networks of phos- phorylated proteins were found. The phosphoproteome of S. pyogenes can provide the theoretical foundation for the deep study on the molecular mechanism of bacterial virulence and antibiotic resistance, and the new view for the development of novel anti-bac- terial drugs.