多浪羊多胎基因全基因组关联分析研究

Whole Genomic Association Analysis of Polyembryonic Gene in Duolang Sheep

利用全基因组重测序技术,对多浪羊产羔性状进行了全基因组关联分析(GWAS),挖掘了绵羊繁殖性状相关候选基因及分子标记,结果表明:Fst检验结果共筛选出135个显著窗口,可以发现大部分的Fst值都集中在0.35~0.50之间。对筛选到的显著窗口进行基因注释,共得到51个基因。再进行富集分析后发现Fst的最大值为0.8710,且位于NCOA1基因内部。在雌配子发生条目中,多胎组和单胎组共同基因为INHBA。同样,利用ENSEMBLE数据库进行基因注释,共有28个基因与显著窗口有交集,如ACP1、ING5、ARNT等。这些候选基因和分子标记的揭示对绵羊繁殖性状功能基因的挖掘具有参考价值和理论意义。

The whole genomic association analysis （GWAS） of lambing traits of Duolang sheep was conducted by using the whole genomic resequencing technique, and the candidate genes and molecular markers related to the breeding traits of sheep were excavated. The Fst test screened out 135 significant windows, and their Fs, value was mostly concentrated within 0.35 - 0.50. A to- tal of 51 genes were obtained by the gene annotation to the selected significant windows. The further enrichment analysis found that the maximum value of Fst was 0.8710, and it was located within the gene NCOA1. In the female gametogenic clauses, the common gene of polyembryonic group and unigerminal group was INHBA. Through using ENSEMBLE database for gene annotation, a total of 28 genes （ such as ACP1, ING5, ARNT, etc. ） had intersection with the significant window. The disclosure of these candidate genes and molecular markers has reference value and theoretical significance for the excavation of functional genes related to sheep reproductive traits.