NFAT2在原发性肝癌中的表达及对肝癌细胞增殖及凋亡的影响

Expression of NFAT2 in hepatocellular carcinoma and its effect on proliferation and apoptosis of hepatocellular carcinoma cells

目的:探讨激活的T细胞核因子2(nuclear factor of activated T cells 2,NFAT2)在原发性肝癌中表达及对肝癌细胞增殖及凋亡影响。方法:选取40例肝癌患者,取其癌组织与癌旁组织,蛋白质印迹检测NFAT2蛋白表达;构建NFAT2过表达质粒并转染肝癌细胞Huh7和PLC,通过CCK8方法和克隆形成实验检测肝癌细胞增殖能力,流式细胞术检测肝癌细胞凋亡,蛋白质印迹检测相关凋亡蛋白表达。结果:NFAT2在肝癌组织中的表达明显低于癌旁组织(P<0.05);NFAT2质粒转染细胞后,肝癌细胞增殖率明显降低(P<0.01),克隆形成数明显减少(P<0.05),凋亡率明显增加(P<0.05),相关凋亡蛋白Caspase-8及Caspase-3表达增加。结论:肝癌组织中NFAT2的表达明显下降,NFAT2过表达可抑制肝癌细胞增殖并促进其凋亡。

Objective：To investigate the expression of the nuclear factor of activated T cells 2（NFAT2）in primary hepatocellular carcinoma（HCC） tissues and its effect on the proliferation and apoptosis of HCC cells.Methods：Total protein was extracted from the cancer and adjacent non-cancerous liver tissues from40 HCC patients and the expression of NFAT2 protein was detected by Western blotting.NFAT2 overexpression plasmid was constructed and was transfected into HCC cell lines Huh7 and PLC.We used CCK8 assay and cell colony formation assay to evaluate the HCC cell proliferation,flow cytometry to evaluate HCC apoptosis and Western blotting to examine apoptosis related proteins.Results：The expression of NFAT2 in HCC tissues was significantly lower than that in the adjacent non-cancerous liver tissues（P 0.05）.After cells were transfected with NFAT2 plasmid,cell proliferation rate and colony formation number significantly decreased（P 0.05）,but apoptosis rate significantly increased（P 0.01）,the expression of the apoptosis-related protein cleaved Caspase-8 and cleaved Caspase-3 was increased.Conclusion：NFAT2 expression in HCC tissues decreased significantly; overexpression of NFAT2 could reduce cell proliferation and promote cell apoptosis.