复合菌同时去除苯胺与氨氮及其影响因素研究

Degrading Aniline and Ammonia-Nitrogen Simultaneously by Compound Bacteria and Studying the Influence Factors

为了实现同时去除苯胺和苯胺降解过程中释放的氨氮,选用苯胺降解菌,假单胞菌Z1(Pseudomonas sp.Z1)和异养硝化菌,不动杆菌Y1(Acinetobacter sp.Y1)进行复合研究,并以单株菌Z1作为对照考察复合菌的降解效果。结果表明,复合菌(Z1+Y1)可以有效地同时去除苯胺和苯胺在降解过程中积累的氨氮。培养48 h后,Z1的苯胺、总氮和COD去除率为99.9%、25.7%和57.2%,氨氮积累量为60 mg/L左右;而复合菌的苯胺、总氮和COD去除率则分别可以达到99.9%、80.1%和88.4%,氨氮积累量仅为5 mg/L左右,有效地同时去除了苯胺和苯胺在降解过程中积累的氨氮。对复合菌进行降解条件优化,最佳复合比例为1∶1,最适碳氮比(C/N)为16,在400~800 mg/L初始苯胺浓度条件下,复合菌都可以有效地同时去除苯胺和苯胺在降解过程中积累的氨氮。

In order to degrade aniline and ammonia released from aniline degradation simultaneously, anilin--degradation bacteria Pseudomonas sp. Z1 was combined with heterotrophic nitrifier Acinetobacter sp.Y1 and degradation effects of mixed bacteria were showed by comparing with the single bacteria Z1. Results indicated that the compound bacteria of Z1 and Y1 were able to degrade aniline and the accumulated ammonia simultaneously. After 48 h cultivation, the degradation rate of aniline, TN and COD by Z1 were 99.9%, 25.7% and 57.2% respectively, and the accumulation of NH4＋-N was about 60 mg/L. Whereas, the removal rate of aniline, TN and COD by the compound bacteria were capable of reaching 99.9%, 80.1% and 88A% respectively, and NH4＋-N remainder was only about 5 mg/L, indicating the compound bacteria were able to degrade aniline and the accumulated ammonia simultaneously. The degradation conditions of the compound bacteria were optimized, with the best proportion of combination as 1：1 and the optimal C/N as 16, in the conditions of 400 mg/L to 800 mg/L aniline concentration, the compound bacteria are able to degrade aniline and the accumulated ammonia simultaneously.