摘要
以PEG /磷酸盐双水相系统萃取BSA为对象 ,研究了双水相系统成相浓度、外加盐NaCl、BSA起始浓度等对BSA在两相间的分配系数和BSA下相萃取率的影响 .结果发现 ,BSA大部分被萃取入双水相体系的下相 ,成相组成为PEG 6 0 0 0 10 % (质量分数 ) /PO3 - 46 % (质量分数 )和PEG 10 0 0 0 6 % (质量分数 ) /PO3 - 46 % (质量分数 )的双水相系统有利于BSA的萃取分配 ;外加NaCl对PEG 6 0 0 0 10 % (质量分数 ) /PO3 - 46 % (质量分数 )系统萃取BSA的影响不大 ;得出了上、下相BSA浓度和BSA在两相间分配系数K与系统BSA起始浓度之间的表达式 。
BSA partitioning was performed in the PEG/phosphate aqueous two-phase system. The effects of the phase compositions concentrations, addition of NaCl and the initial concentration of BSA on the partition coefficient K and the fraction of BSA in the bottom phase Y were investigated. The results showed that BSA was enriched to the bottom phase. The phase compositions of PEG 6000 mass fraction is 10%/PO 3- 4 mass fraction is 6% and PEG 10000 mass fraction is 6%/PO 3- 4 mass fraction is 6% respectively were desirable for partitioning. The addition of NaCl showed no influence on BSA partitioning in PEG 6000 mass fraction is 10% /PO 3- 4 mass fraction is 6% system. The mathematical model for K was derived from the initial mass concentration of BSA and well fitting with the experimental data.
出处
《华南理工大学学报(自然科学版)》
EI
CAS
CSCD
北大核心
2002年第8期64-68,共5页
Journal of South China University of Technology(Natural Science Edition)
基金
广东省自然科学基金资助项目 (0 0 0 45 3)
华南理工大学自然科学基金资助项目~~
关键词
PEG/磷酸盐双水相系统
分配
牛血清白蛋白
PEG/phosphate aqueous two-phase system
partitioning
bovine serum albumin (BSA).