冻干人纤维蛋白黏合剂在围绝经期雌兔干眼的应用研究

Therapetic effects of freeze-dried human fibrin glue on xerophthalmia in perimenopausal female rabbit

目的探究冻干人纤维蛋白黏合剂通过泪道栓塞的方式对围绝经期雌兔干眼症的预防和治疗效果。方法选取72只经抗感染处理后剪除第三眼睑的雌兔,制作围绝经期雌兔干眼症模型,手术完成后将其随机分为6组(每组12只):术后无处理组(A组)、PBS预防组(B组)、冻干人纤维蛋白预防组(C组)、造模后无处理组(造模时间:术后2个月,D组)、PBS治疗组(E组)、冻干人纤维蛋白治疗组(F组),并于注射前及注射后2周、4周及6周行Schirmer实验(SIT)检查、角膜荧光素(fluorescein,FL)染色及角膜共聚焦显微镜检查。结果D、E、F三组不同时间点间的FL评分和SIT值有一定差别(F=27.346、10.608,P=0.000、0.001);三组间FL评分和SIT值有一定差别(F=7.579、6.786,P=0.002、0.007);三组的FL评分和SIT值的变化趋势有明显差别(F=44.897、3.424,P=0.000、0.045)。D组、E组、F组在处理2周、4周、6周后,F组FL评分和SIT值较D组明显好转,差异有统计学意义(t=2.906、3.654、4.504,P=0.022、0.017、0.013;t=4.573、5.759、7.231,P=0.032、0.019、0.008);E组、F组FL评分和SIT值在处理后相比,差异有统计学意义(t=2.776、4.124、5.324,P=0.032、0.026、0.017;t=1.969、3.122、4.324,P=0.038、0.023、0.009)。A、B、C三组注射6周后上皮基底细胞(F=17.306,P=0.002)和炎症细胞密度(F=34.024,P=0.000)比较,D、E、F三组上皮基底细胞(F=3.749,P=0.042)和炎症细胞密度(F=8.806,P=0.005)比较,均有统计学差异。结论通过冻干人纤维蛋白黏合剂进行泪道栓塞对于干眼症的预防和治疗均有显著疗效。

Objective To explore prevention and cure effects of the freeze-dried human fibrin glue as the way of the lacrimal duct embolization on xerophthalmia in perimenopausal female rabbit. Methods A total of 72 female rabbits, after anti infection treatment and were cut off third eyelid, were made into perimenopausal xerophthalmia rabbit models. After surgery, all of these rabbits were randomly divided into 6 groups （12 rabbits per group） ：No treatment group after surgery （group A）, PBS prevention group （group B） ,freeze-dried human fibrin prevention group （group C） ;no treatment group after modeling （ modeling time： Two weeks after surgery, group D） ,PBS treatment group （group E）, freeze-dried human fibrin treatment group （group F）. The Schirmer test （SIT）, corneal fluorescein （FL） and corneal confocal microscope were performed before and 2 weeks,4 weeks,6 weeks after injection. Results There were statistical differences in FL score and SIT in group A, group B and group C among different time points （F = 27.346,10. 608 ;P = 0. 000,0. 001 ） ;There were statistical differences be- tween FL scores and SIT among three groups （F = 7.579,6.786 ; P = 0. 002, 0. 007） ;There was significant difference in FL scores and SIT trends among three groups（F=44. 897,3. 424;P =0. 000,0. 045）. The FL score and SIT of group D, group E and group F were significantly improved after treatment for 2 weeks,4 weeks and 6 weeks, the differences were statistically significant （ t = 2.906,3. 554,4. 504 ;P = 0. 022,0. 017,0.013. t = 4. 573,5. 759,7.231 ;P = 0. 032,0. 019,0. 008 ）;The difference between FL score and SIT in group E and group F was statistically significant after treatment （ t = 2. 776,4. 124, 5.324 ;P = 0. 032,0. 026,0.017. t = 1. 969,3. 122,4. 324 ; P = 0. 038,0. 023, 0. 009）. After injection of 6 weeks,the epithelial basal cells（F = 17. 306 ,P = 0.002 ） and inflammatory cells （ F = 34. 024, P = 0.000 ） of group A, B and C were significant changed, the differences were statistically significant. After injection of 6 weeks, the epithelial basal cells （ F = 3. 749, P = 0. 042 ） and inflammatory cells（F = 8. 806, P = 0. 005 ） of group D, E and F were significant changed, the differences were also statistically significant. Conelusion Lacrimal duct embolization with freeze-dried human fibrin glue is effective for the xerophthalmia.