贝伐单抗在兔膝关节腔内注射的安全性

Safety of Intra-articular Injection of Bevacizumab in the Knee Joint of Rabbit

目的观察贝伐单抗兔膝关节腔内注射的安全性。方法 32只兔随机分为4组:正常对照组和贝伐单抗1,2,4 mg组,各8只。4组分别膝关节腔内注射0.9%氯化钠溶液和贝伐单抗1,2,4 mg,均每3周注射1次,共2次。6周后观察各组生物习性及精神状态,处死动物观察膝关节软骨、滑膜组织病理切片和软骨过碘酸-希夫(AB-PAS)染色切片,并进行血液细胞学、生化检查和Mankin’s评分。结果 4组动物生物习性及精神状态正常,软骨与滑膜组织结构没有明显异常改变;血白细胞、红细胞、血小板、丙氨酸氨基转移酶、尿素及Mankin’s评分均差异无统计学意义。结论贝伐单抗关节腔内注射对兔膝关节及全身未见明显毒性作用。

Objective To evaluate the toxicity of the intra-articular injection of bevacizumab in the knee of the rabbit.Methods Thirty-two rabbits were divided into 3 experimental groups and normal control group.Three experimental groups were received intra-articular injection of bevacizumab （1, 2, 4 mg respectively） once every three weeks for two times and the normal control group was received the same amount of 0.9% sodium chloride solution.The animals were sacrificed after 6 weeks.Blood test was examined before and after treatment.Pathologic examinations of liver, kidney and artiluar tissue were taken after the sacrifice.The hematoxylin and eosin stain for synovium and cartilage were performed.The AB-PAS stain and Mankin＇s scale for cartilage were performed.Results All the rabbits kept normal physiological activity.There was no significant difference of major organs and articular tissue between experimental groups and normal control group.There was no significant difference for WBC, RBC, PLT, ALT, BUN and Mankin＇s scale among all groups.Conclusion No systemic toxicity effects were found for the intra-articular injection of bevacizumab in the knee of the rabbit.