衍生化技术用于生物基质中类固醇类激素分析的研究进展

Progress of Chemical Derivatization in Analysis of Endogenous Anabolic Steroids in Bio-matrices

类固醇类激素在生物体内含量极低,由于其结构中含有羟基、羰基等极性官能团,使其在气相色谱-质谱联用(GC-MS)检测中受到限制;类固醇类激素化学结构中缺少可离子化的官能团,在液相色谱-质谱联用(LC-MS)中检测的灵敏度达不到要求,因此对其进行准确定量具有重要意义。该文具体介绍各类固醇激素分析方法中所采用的衍生化方式以及衍生化条件,并且探讨各种衍生化方法的优缺点。

The content of endogenous anabolic steroids is extremely low in biological matrices.Its chemical structure contains polar functional groups such as hydroxyl and carbonyl, which limits their applicability in GC-MS.The lack of ionized groups in the chemical structure leads to poor sensitivity in LC-MS, which plays a significant role in various physiological activities analysis.It is an effective way to enhance the response of mass spectrometer by modifying the chemical structure of endogenous anabolic steroids through derivatization technology.This review summarizes various derivatization reagents and corresponding derivatization processes of endogenous anabolic steroids analysis in the methods based on different testing instruments and methodologies.The advantage and disadvantage of all kinds of derivatiaztion methods and the prospect of the endogenous anabolic steroids derivatiaztion techniques are also discussed.