HPLC法同时测定清热治痢丸中6种成分

Simultaneous determination of six constituents in Qingre Zhili Pills by HPLC

目的建立HPLC法同时测定清热治痢丸(三颗针和马齿苋)中盐酸小檗碱、盐酸药根碱、盐酸巴马汀、山柰酚、木犀草素、芹菜素的含有量。方法该药物甲醇提取液的分析采用Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm);以乙腈-0.02%磷酸二氢钾为流动相,梯度洗脱;体积流量0.9 m L/min;检测波长265 nm和360 nm;柱温35℃。结果 6种成分分别在各自范围内线性关系良好(r≥0.999 3),平均加样回收率97.16%~99.90%,RSD0.62%~1.48%。结论该方法简便快速,可用于清热治痢丸的质量控制。

AIM To develop an HPLC method for the simultaneous content determination of berberine hydro- chloride, jatrorrhizine hydrochloride, palmatine hydrochloride, kaempferol, luteolin and apigenin in Qingre Zhili Pills （Berberidis Radix and Portulacae Herba）. METHODS The analysis of methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent TC-CI8 column （4.6 mm × 250 mm, 5 μm）, with the mobile phase comprising of acetonitrile-0. 02% potassium dihydrogen phosphate flowing at 0. 9 mL/min in a gradient elution manner, and the detection wavelengths were set at 265 nm and 360 nm. RESULTS Six constituents showed good linear relationships within their own ranges （r≥0. 999 3 ）, whose average recoveries were 97. 16% -99.90% with the RSDs of 0. 62% - 1.48% . CONCLUSION This simple method can be used for the rapid quality control of Qingre Zhili Pills.