柴达木枸杞化学成分的测定

Determination of chemical consituents in Lycii Fructus from Qaidam Basin

目的测定柴达木枸杞Lycii Fructus化学成分的含有量。方法分光光度法测定多糖、总黄酮、类胡萝卜素含有量,HPLC法测定甜菜碱和东莨菪内酯含有量,凯氏定氮法测定蛋白质含有量,再进行主成分分析。结果 6个产地样品中类胡萝卜素、甜菜碱、东莨菪内酯含有量有显著差异(P<0.05),而多糖、总黄酮、蛋白质含有量无显著差异(P>0.05)。3个采摘期样品中各成分含有量也均无显著差异(P>0.05)。德令哈市产样品的主成分综合得分最高,乌兰县产者次之。结论德令哈市产柴达木枸杞的质量最优。

AIM To determine the contents of chemical consituents in Lycii Fructus from Qaidam Basin. METHODS Spectrophotometry was adopted in the content determination of polysaccharides, total flavonoids and carotenoid. HPLC was applied to the content determination of betaine and scopoletin. Kjeldahl method was used for the content determination of protein. Then principal component analysis was performed. RESULTS The contents of carotenoid, betaine and scopoletin in samples from six growing areas showed obvious differences （ P 〈 0. 05 ）, while those of polysaccharides, total flavonoids and protein exhibited no obvious differences （P 〉 0. 05 ）. The con- tents of various constituents in samples at three picking time also had no obvious differences （ P 〉 0. 05 ）. The com- prehensive score of principal components of samples from Delingha City was the highest, followed by that from Ulan County. CONCLUSION The quality of Lycii Fructus from Qaidam Basin from Delingha City is the best.